Mammalian Pol III promoter H1 can transcribe shRNA inducing RNAi in chicken cells.

Molecular Biology Reports
Jing YuanNing Li

Abstract

Double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful reverse genetic tool to silence gene expression in multiple organisms. RNAi based on DNA vector is not sufficiently established in chicken species. The present study was performed to evaluate RNAi induced by shRNA transcribed from mammalian Pol III promoter H1 in the chicken cells by using a dual fluorescence reporter assay, a plasmid encoding GFP and a plasmid encoding RFP. The evaluation of RNAi efficiency was performed in two kinds of chicken cell type: primary CEF cells and chicken DT-40 cells by lipofection. GFP- and RFP-expressing cells were observed under fluorescent microscopy, and their mRNAs content were analyzed by quantitative RT-PCR. The intensity of the green fluorescence generated by GFP was greatly suppressed by human H1 promoter transcribed GFP-shRNA. Quantitative RT-PCR analysis showed that normalized GFP mRNA expression was reduced to 37 and 32 in primary CEF and DT-40 cells, respectively. In contrast to GFP, the intensity of the red fluorescence generated by RFP protein and the RFP mRNA levels remained unchanged. Consequently, it was concluded that the RNAi induced by shRNA transcribed from mammalian Pol III promoter H1 is ap...Continue Reading

References

Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H ReppF Dreyer
May 5, 1997·FEBS Letters·M OkabeY Nishimune
Feb 3, 2000·Nature Cell Biology·F Wianny, M Zernicka-Goetz
Aug 21, 2001·Mechanisms of Ageing and Development·C D Link
Jan 30, 2002·Trends in Biotechnology·Natasha J Caplen
Feb 12, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Zhiyuan GongTie Yan
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Guangchao SuiYang Shi
Jul 12, 2002·Nature·Gregory J Hannon
Aug 15, 2002·Current Biology : CB·Wen-Yuan HuFrederic D Bushman
Dec 24, 2002·Nature Biotechnology·Vladimir PekarikEsther T Stoeckli
Feb 1, 2003·Nature Reviews. Genetics·William R A BrownStuart A Wilson
Jan 27, 2004·BioTechniques·Shin-ichiro KojimaGary G Borisy
Mar 3, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Catherine E Krull
Jun 2, 2004·Genesis : the Journal of Genetics and Development·Catherine Chesnutt, Lee Niswander

❮ Previous
Next ❯

Citations

Mar 6, 2013·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Kamran HaqShayan Sharif

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Experimental Zoology. Part A, Comparative Experimental Biology
Fuminori SatoMasa-aki Hattori
Proceedings of the National Academy of Sciences of the United States of America
Guangchao SuiYang Shi
© 2022 Meta ULC. All rights reserved