Mammalian Solute Carrier (SLC)-like transporters of Legionella pneumophila

Scientific Reports
Ashley BestYousef Abu Kwaik

Abstract

Acquisition of nutrients during intra-vacuolar growth of L. pneumophila within macrophages or amoebae is poorly understood. Since many genes of L. pneumophila are acquired by inter-kingdom horizontal gene transfer from eukaryotic hosts, we examined the presence of human solute carrier (SLC)-like transporters in the L. pneumophila genome using I-TASSER to assess structural alignments. We identified 11 SLC-like putative transporters in L. pneumophila that are structurally similar to SLCs, eight of which are amino acid transporters, and one is a tricarboxylate transporter. The two other transporters, LstA and LstB, are structurally similar to the human glucose transporter, SLC2a1/Glut1. Single mutants of lstA or lstB have decreased ability to import, while the lstA/lstB double mutant is severely defective for uptake of glucose. While lstA or lstB single mutants are not defective in intracellular proliferation within Acanthamoeba polyphaga and human monocyte-derived macrophages, the lstA/lstB double mutant is severely defective in both host cells. The two phenotypic defects of the lstA/lstB double mutant in uptake of glucose and intracellular replication are both restored upon complementation of either lstA or lstB. Our data show t...Continue Reading

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Citations

Aug 14, 2019·Infection and Immunity·Richard C WhiteNicholas P Cianciotto
Apr 28, 2021·International Journal of Medical Microbiology : IJMM·Mareike KunzeWolfgang Eisenreich

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Methods Mentioned

BETA
PCR

Software Mentioned

Iterative Threading Assembly Refinement ( I
BLAST
- TASSER )
align
TM
TASSER

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