Mammalian testis: a target of in vivo electroporation

Development, Growth & Differentiation
Kentaro Yomgogida

Abstract

Mammalian spermatogenesis consists of three biologically significant processes: stem cell self-renewal and differentiation, meiosis, and haploid cell morphogenesis. Understanding the molecular mechanisms behind these processes might provide clues to the puzzle of species preservation and evolution, and to treatments for male infertility. However, few useful in vitro systems exist to investigate these processes at present. To elucidate these mechanisms, in vivo electroporation of the testis might be a convenient option. Since DNA solution can be injected into the seminiferous tubule via the rete testis, similar to germ cell transplantation, it is easy to transfect expression vectors into various differentiated germ cells and supporting Sertoli cells with adequate electric shock. Unfortunately, it is difficult to create transgenic animals using this method because of its low efficiency. However, gain- and loss-of-function assays, promoter assays, and tagged-protein behavior assays can be conducted with this technique, as in in vitro culture systems.

References

Jan 5, 2000·The Journal of Experimental Zoology·Y YamazakiK Imoto
Aug 24, 2002·Biology of Reproduction·Kentaro YomogidaYoshitake Nishimune
Jun 14, 2005·Developmental Biology·Masanobu ShojiNorio Nakatsuji
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Jun 8, 2007·Nature·Keisuke OkitaShinya Yamanaka
Jun 21, 2007·Reproductive Biomedicine Online·Alberto FerlinCarlo Foresta
Jul 13, 2007·Stem Cell Reviews·M William LenschThorsten M Schlaeger

Citations

Dec 2, 2009·Cell Biology and Toxicology·Dominic J Wells
Aug 1, 2009·Development, Growth & Differentiation·Morris BeshayRalph A Schmid

Related Concepts

Gametes
Haploid Cell
M Phase, Meiotic
Structure of Sertoli Cell
Spermatocytes
Spermiogenesis
Spermatogonia
Colony-forming Unit
Testis
Developmental Biology

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