Abstract
Bone morphogenetic protein 1 (BMP1) is the prototype of a subgroup of metalloproteinases with manifold roles in morphogenesis. Four mammalian subgroup members exist, including BMP1 and mammalian Tolloid-like 1 (mTLL1). Subgroup members have a conserved protein domain structure: an NH2-terminal astacin-like protease domain, followed by a fixed order of CUB and epidermal growth factor-like protein-protein interaction motifs. Previous structure/function studies have documented those BMP1 protein domains necessary for secretion, and activity against various substrates. Here we demonstrate that, in contradiction to previous reports, the most NH2-terminal CUB domain (CUB1) is not required for BMP1 secretion nor is the next CUB domain (CUB2) required for enzymatic activity. The same is true for mTLL1. In fact, secreted protease domains of BMP1 and mTLL1, devoid of CUB or epidermal growth factor-like domains, have procollagen C-proteinase (pCP) activity and activity for biosynthetic processing of biglycan, the latter with kinetics superior to those of the full-length proteins. Structure-function analyses herein also suggest differences in the functional roles played by some of the homologous domains in BMP1 and mTLL1. Surprisingly, alt...Continue Reading
References
Nov 1, 1991·Cell·M J ShimellM B O'Connor
Dec 8, 1989·European Journal of Biochemistry·E Kessler, R Adar
Jul 1, 1986·Collagen and Related Research·E KesslerR Niece
Jul 1, 1995·Protein Science : a Publication of the Protein Society·J S Bond, R J Beynon
May 20, 1993·Journal of Molecular Biology·P Bork, G Beckmann
Jan 19, 1996·Science·E KesslerD S Greenspan
Jun 1, 1996·Genomics·K TakaharaD S Greenspan
Sep 15, 1996·The Biochemical Journal·P J RoughleyJ S Mort
Feb 1, 1997·Development, Growth & Differentiation·J J LinM Maéno
Sep 10, 1999·Developmental Biology·I C ScottD S Greenspan
Mar 22, 2000·Biochemistry·A L SieronW Stöcker
Jul 18, 2000·The Journal of Biological Chemistry·I C ScottD S Greenspan
Mar 22, 2001·Nature·I C ScottD S Greenspan
Jul 10, 2002·The Journal of Biological Chemistry·Sylvie Ricard-BlumDavid J S Hulmes
Oct 24, 2002·The Journal of Biological Chemistry·Barry M SteiglitzDaniel S Greenspan
Dec 5, 2002·Mechanisms of Development·Leslie DaleShelley A Goodman
Dec 11, 2002·The Journal of Biological Chemistry·Dallas P VeitchM Peter Marinkovich
Mar 15, 2003·The Journal of Biological Chemistry·Nichola HartiganKarl E Kadler
Jun 17, 2003·Molecular and Cellular Biology·William N PappanoDaniel S Greenspan
Sep 24, 2004·The Journal of Biological Chemistry·Laure Garrigue-AntarKarl E Kadler
Apr 9, 2005·The Journal of Biological Chemistry·Vasiliki PetropoulouKarl E Kadler
Apr 19, 2005·The Journal of Biological Chemistry·Catherine MoaliDavid J S Hulmes
May 6, 2005·Development·Mihaela SerpeMichael B O'Connor
Jul 1, 2005·Molecular and Cellular Biology·Gaoxiang GeDaniel S Greenspan
Citations
Dec 17, 2008·Nature Cell Biology·Koichi KobayashiThomas N Sato
May 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Richard BerryClair Baldock
Jul 21, 2009·The Journal of Biological Chemistry·Guorui HuangDaniel S Greenspan
Oct 6, 2009·The Journal of Biological Chemistry·Daniel KronenbergCatherine Moali
Mar 9, 2010·The Journal of Biological Chemistry·Mourad BekhoucheCatherine Moali
Oct 27, 2011·The Journal of Biological Chemistry·Alison Muir, Daniel S Greenspan
Sep 14, 2011·Circulation·Sharon CresciUNKNOWN BARI 2D Study Group
May 23, 2007·Biological Chemistry·Carsten WermterWalter Stöcker
Jan 2, 2010·FEBS Letters·Richard BerryClair Baldock
Feb 24, 2016·Scientific Reports·Christopher P BayleyClair Baldock
Oct 21, 2009·Journal of Tissue Engineering and Regenerative Medicine·Rosmarie HardmeierStefan Marlovits
Jan 9, 2007·Biochimica Et Biophysica Acta·Xunhao XiongRongqing Zhang
Oct 15, 2013·The Biochemical Journal·Romain SalzaSylvie Ricard-Blum
Jan 12, 2008·Reproductive Sciences·Brenda C Timmons, Mala Mahendroo
Nov 10, 2018·FEBS Letters·Qin ZhuGuo-Ru Huang
Mar 19, 2011·Journal of the American Society of Nephrology : JASN·Hirokazu Okada
Apr 21, 2007·The Journal of Biological Chemistry·Guillaume BlancCatherine Moali
Jul 31, 2008·The Journal of Biological Chemistry·Timothy J Geach, Leslie Dale
Oct 2, 2019·European Journal of Human Genetics : EJHG·Talal AlanziFowzan S Alkuraya
Jul 16, 2016·Circulation. Cardiovascular Genetics·Stephanie LaHayeVidu Garg
Mar 19, 2021·Frontiers in Physiology·Gege ZhangWenguang Liu
Aug 27, 2021·Matrix Biology Plus·Priscillia LagoutteCatherine Moali