Management of carbon tetrachloride-induced acute liver injury in rats by syngeneic hepatocyte transplantation in spleen and peritoneal cavity

World Journal of Gastroenterology : WJG
Charalampos PilichosIsmini Donta

Abstract

Acute hepatitis may seldom have a fulminant course. In the treatment of this medical emergency, potential liver support measure must provide immediate and sufficient assistance to the hepatic function. The goal of our study was to study the adequacy of hepatocyte transplantation (HCTx) in two different anatomical sites, splenic parenchyma and peritoneal cavity, in a rat model of reversible acute hepatitis induced by carbon tetrachloride (CCl(4)). After CCl(4) intoxication, 84 male Wistar rats used as recipients were divided in to four experimental groups accordingly to their treatment: Group A (n=24): intrasplenic transplantation of 10x10(6) isolated hepatocytes, Group B (n=24): intraperitoneal transplantation of 20x10(6) isolated hepatocytes attached on plastic microcarriers, Group C (n=18): intrasplenic injection of 1 mL normal saline (sham-operated controls), Group D (n=18): intraperitoneal injection of 2.5 mL normal saline (sham-operated controls). Survival, liver function tests (LFT) and histology were studied in all four groups, on d 2,5 and 10 post-HCTx. The ten-day survival (and mean survival) in the 4 groups was 72.2% (8.1+/-3.1), 33.3% (5.4+/-3.4), 0% (3.1+/-1.3) and 33.3% (5.4+/-3.6) in groups A, B, C, D, respectivel...Continue Reading

References

Jan 1, 1991·Virchows Archiv. A, Pathological Anatomy and Histopathology·D Henne-BrunsB Kremer
Jan 1, 1988·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·J P VroemenG Kootstra
Sep 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A A DemetriouJ R Chowdhury
Oct 1, 1980·The Journal of Surgical Research·B G SommerJ S Najarian
Aug 1, 1995·Journal of Biomedical Materials Research·D J MooneyR Langer
May 30, 2001·Transplantation Proceedings·C FotiadisM Sechas

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Citations

Dec 4, 2015·Toxicology and Applied Pharmacology·Michaël MaesHartmut Jaeschke
Nov 4, 2008·Phytotherapy Research : PTR·Chia-Yu ChangJoe-Sharg Li
Jun 19, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Anuradha KrishnanMichael Charlton
Jul 14, 2012·Journal of Internal Medicine·C JornsB G Ericzon
Apr 11, 2014·CNS Neuroscience & Therapeutics·Wieslawa Agnieszka FogelMiroslaw Mussur
May 13, 2006·International Journal of Radiation Oncology, Biology, Physics·Hans ChristiansenHeinz Schmidberger
Mar 2, 2012·Molecular Biology of the Cell·Frauke GoemanGiulia Piaggio
Jan 10, 2018·World Journal of Transplantation·Ryan D HeathVeysel Tahan
Mar 23, 2006·World Journal of Gastroenterology : WJG·Xiao-Fei ZhouAi-Lian Liu

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