Mandible exosomal ssc-mir-133b regulates tooth development in miniature swine via endogenous apoptosis

Bone Research
Ye LiSonglin Wang

Abstract

Signal transduction between different organs is crucial in the normal development of the human body. As an important medium for signal communication, exosomes can transfer important information, such as microRNAs (miRNAs), from donors to receptors. MiRNAs are known to fine-tune a variety of biological processes, including maxillofacial development; however, the underlying mechanism remains largely unknown. In the present study, transient apoptosis was found to be due to the expression of a miniature swine maxillofacial-specific miRNA, ssc-mir-133b. Upregulation of ssc-mir-133b resulted in robust apoptosis in primary dental mesenchymal cells in the maxillofacial region. Cell leukemia myeloid 1 (Mcl-1) was verified as the functional target, which triggered further downstream activation of endogenous mitochondria-related apoptotic processes during tooth development. More importantly, mandible exosomes were responsible for the initial apoptosis signal. An animal study demonstrated that ectopic expression of ssc-mir-133b resulted in failed tooth formation after 12 weeks of subcutaneous transplantation in nude mice. The tooth germ developed abnormally without the indispensable exosomal signals from the mandible.

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Citations

Mar 13, 2020·Cell Proliferation·Xiaoru ShaoYunfeng Lin
Feb 6, 2020·Frontiers in Physiology·Lyndon F CooperMiya Kang
Jul 30, 2020·International Journal of Molecular Sciences·Sanjiv NeupaneJae-Young Kim
Sep 17, 2020·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Rui ShengQuan Yuan
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Jul 6, 2021·Frontiers in Cell and Developmental Biology·John AbramyanMarcela Buchtová

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Methods Mentioned

BETA
transfection
flow cytometry
PCR
transmission electron microscopy
protein assay
electrophoresis

Software Mentioned

PicTar
TargetScan

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