PMID: 42641Dec 1, 1979

Manganese, an essential trace element for N2 fixation by Rhodospirillum rubrum and Rhodopseudomonas capsulata: role in nitrogenase regulation

Journal of Bacteriology
D C Yoch

Abstract

Nitrogenase (N(2)ase) from the photosynthetic bacterium Rhodospirillum rubrum can exist in two forms, an unregulated form (N(2)ase A) and a regulatory form (N(2)ase R), the latter being identified in vitro by its need for activation by a Mn(2+)-dependent N(2)ase activating system. The physiological significance of this Mn(2+)-dependent N(2)ase activating system was suggested here by observations that growth of R. rubrum and Rhodopseudomonas capsulata on N(2) gas (a condition that produces active N(2)ase R) required Mn(2+), but growth on ammonia or glutamate did not. Manganese could not be shown to be required for the biosynthesis of either nitrogenase or glutamine synthetase or for glutamine synthetase turnover, but it was required for the in vitro activation of N(2)ases from N(2) and glutamate-grown R. rubrum and R. capsulata cells. Chromatium N(2)ase, in contrast, was always fully active and did not require Mn(2+) activation, suggesting that only the purple nonsulfur bacteria are capable of controlling their N(2)ase activity by this new type of regulatory system. Although R. rubrum could not substitute Fe(2+) for Mn(2+) in the in vivo N(2) fixation process, Fe(2+) and, to a lesser extent, Co(2+) could substitute for Mn(2+) in...Continue Reading

Citations

Jan 1, 1987·Critical Reviews in Microbiology·P C Hallenbeck
Jan 1, 1986·Critical Reviews in Microbiology·F Archibald
Oct 1, 1982·European Journal of Biochemistry·H HaakerC Veeger
Feb 1, 1985·Archives of Microbiology·J W Gotto, D C Yoch
Nov 30, 2018·World Journal of Microbiology & Biotechnology·Natalia Akentieva

Related Concepts

Ammonia
Enzyme Activation
Glutamic Acids
Glutamate-Ammonia Ligase
Manganese
Diazotrophy
Vanadium Nitrogenase
Rhodospirillum
Rhodospirillum rubrum

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