Manganese antagonizes iron blocking mitochondrial aconitase expression in human prostate carcinoma cells

Asian Journal of Andrology
Ke-Hung TsuiHorng-Heng Juang

Abstract

To investigate the possible role of manganese in the regulation of mitochondrial aconitase (mACON) activity human prostate carcinoma cell line PC-3 cells. The mACON enzymatic activities of human prostate carcinoma cell line PC-3 cells were determined using a reduced nicotinamide adenine dinucleotide-coupled assay. Immunoblot and transient gene expression assays were used to study gene expression of the mACON. The putative response element for gene expression was identified using reporter assays with site-directed mutagenesis and electrophoretic mobility-shift assays. In vitro study revealed that manganese chloride (MnCl2) treatment for 16 h inhibited the enzymatic activity of mACON, which induced the inhibition of citrate utility and cell proliferation of PC-3 cells. Although results from transient gene expression assays showed that MnCl2 treatment upregulated gene translation by approximately 5-fold through the iron response element pathway, immunoblot and reporter assays showed that MnCl2 treatments inhibited protein and gene expression of mACON. This effect was reversed by co-treatment with ferric ammonium citrate. Additional reporter assays with site-directed mutagenesis and electrophoretic mobility-shift assays suggested t...Continue Reading

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Citations

Oct 20, 2011·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Farida Louise AssemLeonard Stephen Levy
Nov 4, 2008·Biochemistry. Biokhimii︠a︡·L V Matasova, T N Popova

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