Manganese-Dependent Cleavage of Nonphenolic Lignin Structures by Ceriporiopsis subvermispora in the Absence of Lignin Peroxidase.

Applied and Environmental Microbiology
K A JensenKenneth E Hammel

Abstract

Many ligninolytic fungi appear to lack lignin peroxidase (LiP), the enzyme generally thought to cleave the major, recalcitrant, nonphenolic structures in lignin. At least one such fungus, Ceriporiopsis subvermispora, is nevertheless able to degrade these nonphenolic structures. Experiments showed that wood block cultures and defined liquid medium cultures of C. subvermispora rapidly depolymerized and mineralized a (sup14)C-labeled, polyethylene glycol-linked, high-molecular-weight (beta)-O-4 lignin model compound (model I) that represents the major nonphenolic structure of lignin. The fungus cleaved model I between C(inf(alpha)) and C(inf(beta)) to release benzylic fragments, which were shown in isotope trapping experiments to be major products of model I metabolism. The C(inf(alpha))-C(inf(beta)) cleavage of (beta)-O-4 lignin structures to release benzylic fragments is characteristic of LiP catalysis, but assays of C. subvermispora liquid cultures that were metabolizing model I confirmed that the fungus produced no detectable LiP activity. Three results pointed, instead, to the participation of a different enzyme, manganese peroxidase (MnP), in the degradation of nonphenolic lignin structures by C. subvermispora. (i) The degra...Continue Reading

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Citations

Sep 26, 2001·Applied and Environmental Microbiology·M HofrichterA Hatakka
Jul 9, 2004·Applied and Environmental Microbiology·Anderson GuerraJohn Ralph
Jul 30, 2008·FEMS Microbiology Reviews·Ri-He PengQuan-Hong Yao
Feb 16, 2010·Applied Microbiology and Biotechnology·Takahito WatanabeTakashi Watanabe
Mar 23, 2012·The Journal of Biological Chemistry·Elena Fernández-FueyoAngel T Martínez
Dec 20, 2002·Canadian Journal of Microbiology·Vishal Shah, Frantisek Nerud
Mar 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Elena Fernandez-FueyoDan Cullen
Jun 17, 1999·FEBS Letters·C Höfer, D Schlosser
Mar 2, 2002·Applied and Environmental Microbiology·Ana GutiérrezAngel T Martínez
Feb 18, 2011·Organic & Biomolecular Chemistry·Yasunori OhashiTakashi Watanabe
Nov 24, 1999·FEBS Letters·A N KapichK E Hammel
Dec 14, 2005·Applied and Environmental Microbiology·M HofrichterW Fritsche
Dec 26, 2001·Chemical Reviews·R ten Have, P J Teunissen
Jan 15, 2017·Microbiology Spectrum·Miia R MäkeläKristiina Hildén
Jan 12, 2013·ACS Chemical Biology·Rahul SinghLindsay D Eltis
Dec 23, 2008·Biotechnology Advances·Carmen Sánchez

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