Manganese-enhanced magnetic resonance imaging of hypoxic-ischemic brain injury in the neonatal rat

NeuroImage
Marius WiderøeChristian Brekken

Abstract

Hypoxic-ischemic injury (HI) to the neonatal brain results in delayed neuronal death with accompanying inflammation for days after the initial insult. The aim of this study was to depict delayed neuronal death after HI using Manganese-enhanced MRI (MEMRI) and to evaluate the specificity of MEMRI in detection of cells related to injury by comparison with histology and immunohistochemistry. 7-day-old Wistar rat pups were subjected to HI (occlusion of right carotid artery and 8% O(2) for 75 min). 16 HI (HI+Mn) and 6 sham operated (Sham+Mn) pups were injected with MnCl(2) (100 mM, 40 mg/kg) and 10 HI-pups (HI+Vehicle) received NaCl i.p. 6 h after HI. 3D T(1)-weighted images (FLASH) and 2D T(2)-maps (MSME) were acquired at 7 T 1, 3 and 7 days after HI. Pups were sacrificed after MR-scanning and brain slices were cut and stained for CD68, GFAP, MAP-2, Caspase-3 and Fluorojade B. No increased manganese-enhancement (ME) was detectable in the injured hemisphere on day 1 or 3 when immunohistochemistry showed massive ongoing neuronal death. 7 days after HI, increased ME was seen on T(1)-w images in parts of the injured cortex, hippocampus and thalamus among HI+Mn pups, but not among HI+Vehicle or Sham+Mn pups. Comparison with immunohistoc...Continue Reading

References

Feb 1, 1981·Annals of Neurology·J E RiceJ B Brierley
Feb 16, 1995·Brain Research. Developmental Brain Research·A McRaeH Hagberg
Dec 31, 1997·Pediatrics·R C Vannucci, J M Perlman
Mar 20, 1998·Stroke; a Journal of Cerebral Circulation·T Kristián, B K Siesjö
Apr 25, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·W ZhengQ Zhao
Oct 13, 2001·Developmental Neuroscience·R GeddesS J Vannucci
Nov 6, 2002·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Takashi WatanabeThomas Michaelis
Dec 31, 2002·Brain Research. Brain Research Reviews·Atsushi Takeda
Jun 20, 2003·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Ichio AokiShoji Naruse
Dec 19, 2003·Annals of Neurology·Masayuki FujiokaBo K Siesjö
Aug 10, 2004·The Journal of Experimental Biology·Susan J Vannucci, Henrik Hagberg
Nov 5, 2004·The New England Journal of Medicine·Donna M Ferriero
Dec 24, 2004·NMR in Biomedicine·Alan P Koretsky, Afonso C Silva
Feb 8, 2005·Seminars in Perinatology·Claire McLean, Donna Ferriero
Mar 12, 2005·NMR in Biomedicine·Youssef Zaim WadghiriDaniel H Turnbull
Jun 22, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Ralph WeberMathias Hoehn
Jul 1, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Susanne WegenerMathias Hoehn
Jul 28, 2005·Developmental Neuroscience·Robert C Vannucci, Susan J Vannucci
Oct 12, 2005·Brain Research. Brain Research Reviews·Frances J NorthingtonLee J Martin
Oct 27, 2005·Journal of Biochemistry and Molecular Biology·Seok Joon WonByoung Joo Gwag

❮ Previous
Next ❯

Citations

Oct 27, 2009·Pediatric Surgery International·Montserrat BretJuan A Tovar
Dec 13, 2012·European Journal of Applied Physiology·Marianne B HavnesAndreas Møllerløkken
Jun 5, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Aditya N BadeYutong Liu
Nov 7, 2013·Stem Cells International·Abhishek Sohni, Catherine M Verfaillie
Dec 1, 2011·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Oliviero L GobboMarc Dhenain
Mar 31, 2015·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Yohan van de LooijStéphane V Sizonenko
May 16, 2012·NeuroImage·Evan LutkenhoffJ David Jentsch
Jun 23, 2016·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Katherine E OlsonYutong Liu
Oct 21, 2016·The British Journal of Radiology·Tae Yeon JeonJung Hee Lee
Mar 31, 2010·Cell Transplantation·Annelies CrabbeUwe Himmelreich
May 23, 2013·Cancer Research·Shigeyoshi SaitoIchio Aoki

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.