Manganese superoxide dismutase modulates hypoxia-inducible factor-1 alpha induction via superoxide.

Cancer Research
Suwimol KaewpilaL W Oberley

Abstract

Hypoxia-inducible factor 1 (HIF-1) is a transcription factor that plays an important role in O(2) homeostasis. Numerous observations suggest that changes in reactive oxygen species affect HIF-1 alpha stabilization and HIF-1 alpha transcriptional activation in many cell types. The antioxidant enzyme manganese superoxide dismutase (MnSOD) modulates the cellular redox environment by converting superoxide (O(2)(*-)) to hydrogen peroxide and dioxygen. Previous results from our group have shown that overexpression of MnSOD in MCF-7 cells alters stabilization of HIF-1 alpha under hypoxic conditions; however, the underlying mechanism(s) is not known. Here, we tested the hypothesis that MnSOD regulates the expression of HIF-1 alpha by modulating the steady-state level of O(2)(*-). We found that decreasing MnSOD with small interfering RNA in MCF-7 cells resulted in (a) an associated increase in the hypoxic accumulation of HIF-1 alpha immunoreactive protein, (b) a significant increase in the levels of O(2)(*-) (P < 0.01), but (c) no significant change in the steady-state level of H(2)O(2). Removal of O(2)(*-) using spin traps (alpha-4-pyridyl-1-oxide-N-tert-butylnitrone and 5,5-dimethyl-1-pyrroline N-oxide) or the O(2)(*-) scavenger Tempo...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M C KrishnaA Russo
Jan 1, 1991·Free Radical Research Communications·D K St Clair, L W Oberley
Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·G L WangG L Semenza
Jan 20, 1995·The Journal of Biological Chemistry·G L Wang, G L Semenza
Feb 1, 1993·Free Radical Biology & Medicine·P C PanusB A Freeman
Feb 26, 1999·The Journal of Biological Chemistry·P J KallioL Poellinger
Dec 28, 1999·Annual Review of Cell and Developmental Biology·G L Semenza
Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Clara SchroedlNavdeep S Chandel
Sep 8, 2004·Experimental Cell Research·Ingrid NilssonStefan Wennström
Jul 30, 2005·Journal of Cellular Biochemistry·Liang-Yo YangChwen-Ming Shih
Aug 2, 2005·Cell Metabolism·Joslyn K BrunelleNavdeep S Chandel
Dec 14, 2006·Free Radical Research·Isabel L JacksonZeljko Vujaskovic
Apr 28, 2007·Free Radical Biology & Medicine·Benjamin P SouleJames B Mitchell

❮ Previous
Next ❯

Citations

Dec 3, 2009·Antioxidants & Redox Signaling·Sergio Rosales-CorralGabriela Lopez-Armas
Jul 25, 2009·Cellular and Molecular Life Sciences : CMLS·Frank Weinberg, Navdeep S Chandel
Jan 16, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jennifer D SislerYong Qian
Jul 23, 2015·Cancer Chemotherapy and Pharmacology·Tijana StankovićMilica Pešić
Mar 19, 2014·Redox Biology·H Susana MarinhoFernando Antunes
Jan 16, 2017·Free Radical Biology & Medicine·Sehamuddin GaladariFaisal Thayyullathil
Feb 6, 2018·Clinical & Experimental Metastasis·Justin G WilkesJoseph J Cullen
Mar 17, 2010·Journal of Cellular Physiology·María I RosónBelisario E Fernández
Nov 7, 2019·Proceedings of the National Academy of Sciences of the United States of America·Anita B Hjelmeland, Rakesh P Patel
Apr 3, 2020·Antioxidants & Redox Signaling·Cassandra M Moshfegh, Adam J Case
Jun 16, 2017·Journal of Bioenergetics and Biomembranes·Xianghui ZouYueming Zhu
Dec 30, 2014·Journal of Cellular Biochemistry·Shahrzad MovafaghKim Vo
Jul 27, 2017·Frontiers in Cell and Developmental Biology·Rui BenfeitasAdil Mardinoglu
Oct 9, 2019·Proceedings of the National Academy of Sciences of the United States of America·Chenxia HeMarcelo G Bonini
Oct 5, 2019·Oxidative Medicine and Cellular Longevity·Qiaohong QianJianchun Wu
Jun 23, 2020·Oxidative Medicine and Cellular Longevity·Damian GajeckiAdrian Doroszko
Jan 16, 2021·Free Radical Biology & Medicine·Danchen WuStephen L Archer
Jan 24, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chrystalla MytidouLeonidas A Phylactou
Nov 4, 2020·Free Radical Biology & Medicine·Malgorzata KucinskaMarek Murias
Dec 28, 2011·Fertility and Sterility·Valerie I ShavellMichael P Diamond
Apr 6, 2021·Oxidative Medicine and Cellular Longevity·Ines Batinic-HaberleAngeles Alvarez Secord
Apr 24, 2021·Free Radical Biology & Medicine·Romain CarcyDidier F Pisani
Dec 4, 2020·Free Radical Biology & Medicine·Janine Hertzog Santos
Feb 25, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zahra Ghanbari MovahedKamran Mansouri
May 30, 2009·Free Radical Biology & Medicine·Lu Miao, Daret K St Clair
Dec 2, 2009·Seminars in Cell & Developmental Biology·Sajni JossonRuoxiang Wang
Oct 22, 2021·Frontiers in Endocrinology·Theresa QuintingTimm Schreiber
Oct 8, 2020·Cell Metabolism·Calvin S CarterVal C Sheffield
Nov 7, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Duale AhmedEdana Cassol
May 31, 2011·Gynecologic Oncology·Zhongliang JiangGhassan M Saed

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.