Mangiferin inhibits apoptosis and oxidative stress via BMP2/Smad-1 signaling in dexamethasone-induced MC3T3-E1 cells

International Journal of Molecular Medicine
Ling-Zhi DingShi-Hong Chen

Abstract

Mangiferin is a xanthone glucoside, which possesses antioxidant, antiviral, antitumor and anti-inflammatory functions, and is associated with gene regulation. However, it remains unknown whether mangiferin protects osteoblasts, such as the MC3T3-E1 cell line, against glucocorticoid-induced damage. In the present study, MC3T3-E1 cells were treated with dexamethasone (Dex), which is a well-known synthetic glucocorticoid, in order to establish a glucocorticoid-induced cell injury model. After Dex and/or mangiferin treatment, cell viability, apoptosis and reactive oxygen species (ROS) production was measured by Cell Counting kit-8 (CCK-8) and flow cytometry, respectively, and the concentration of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and macrophage colony-stimulating factor (M-CSF) was measured by ELISA. The expression of bone morphogenetic protein 2 (BMP2), phosphorylated‑SMAD family member 1 (p-Smad-1), t-Smad-1, osterix (OSX), osteocalcin (OCN), osteoprotegerin (OPG), receptor activator of nuclear factor-κB (RANK), RANK ligand (RANKL), B‑cell lymphoma 2 (Bcl-2) and Bcl‑2‑associated X protein (Bax) was measured by real-time PCR and/or western blot analysis. The results indicated that pretreatment of MC3T3-E1 cells wit...Continue Reading

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Citations

Sep 24, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Qi ShangHui Ren
Aug 8, 2018·Molecular and Cellular Biochemistry·Anil Kumar SahuJagriti Bhatia
Feb 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zheling FengLigen Lin
Jun 3, 2021·Nutrients·Wojciech SłupskiBeata Nowak

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Methods Mentioned

BETA
PCR
transfection
flow cytometry
ELISA
Protein Assay

Software Mentioned

ImageJ
GraphPad
GraphPad Prism

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