Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening

Frontiers in Plant Science
Mitzuko Dautt-CastroMaria A Islas-Osuna

Abstract

Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. "Kent" was done to identify key genes associated with fruit ripening. Using the Illumina sequencing platform, 67,682,269 clean reads were obtained and a transcriptome of 4.8 Gb. A total of 33,142 coding sequences were predicted and after functional annotation, 25,154 protein sequences were assigned with a product according to Swiss-Prot database and 32,560 according to non-redundant database. Differential expression analysis identified 2,306 genes with significant differences in expression between mature-green and ripe mango [1,178 up-regulated and 1,128 down-regulated (FDR ≤ 0.05)]. The expression of 10 genes evaluated by both qRT-PCR and RNA-seq data was highly correlated (R = 0.97), validating the differential expression data from RNA-seq alone. Gene Ontology enrichment analysis, showed significantly represented terms associated to fruit ripening like "cell wall," "carbohydrate catabolic process" and "starch and sucrose metabolic pr...Continue Reading

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Mar 20, 2016·Plant Science : an International Journal of Experimental Plant Biology·Patrick WinterhagenJens N Wünsche
Jan 18, 2018·Genome Génome / Conseil National De Recherches Canada·Anju BajpaiN K Singh
May 6, 2017·Frontiers in Plant Science·David N KuhnAmir Sherman
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Apr 29, 2020·PloS One·Mohammad Sadat-HosseiniKourosh Vahdati
Feb 24, 2021·BMC Plant Biology·UNKNOWN Mango Genome ConsortiumHaidong Yan
Apr 13, 2019·Journal of Agricultural and Food Chemistry·Jeehye SungYu Wang

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Datasets Mentioned

BETA
SRP045880

Methods Mentioned

BETA
RNA-seq
electrophoresis
PCR

Software Mentioned

BLASTp
Trinity
BLASTx
InterProScan
NCSS
Blast2GO
FastQC
edgeR
run
Bowtie aligner

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