Manifestation of diabetes mellitus on mouse follicular and pre-embryo development: effect of hyperglycemia per se

Metabolism: Clinical and Experimental
M P DiamondA H DeCherney

Abstract

Animal models of diabetes mellitus during pregnancy have repeatedly suggested that maternal hyperglycemia was teratogenic during organogenesis, and thus may contribute to diabetic teratogenesis. However, little attention has been focused on the effects of hyperglycemia on pre-organogenic development. In this report, we examine the effect of hyperglycemia (950 mg glucose/dL) on the development of mouse pre-embryos in vitro. B6C3F1 mice were superovulated with 5 U pregnant mare serum gonadotropin (PMSG) followed by 5 U human chorionic gonadotropin (hCG) 48 hours later. Two cell pre-embryos were recovered 48 hours later, pooled together, and randomly assigned to different treatment groups. Cultures were performed in HAM's F-10 media (Gibco, Long Island, NY) with 0.1% bovine serum albumin (BSA; Sigma, St. Louis, MO) BSA at 37 degrees C in an atmosphere of 5% CO2, 5% O2, and 90% N2 with 15 to 30 embryos per milliliter of culture fluid. Cultures were viewed daily at 24, 48, and 72 hours after culturing, with recording of the development. Compared with control pre-embryos (n = 216), embryos cultured in elevated glucose levels (950 mg/dL) (n = 226) demonstrated marked growth retardation as assessed both by (1) distribution of developme...Continue Reading

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Citations

Apr 1, 1995·Diabetes/metabolism Reviews·U J Eriksson
Apr 1, 2009·Apoptosis : an International Journal on Programmed Cell Death·James H ChappellMary R Loeken
Mar 1, 1996·Obstetrics and Gynecology Clinics of North America·E A Reece, U J Eriksson
Nov 30, 2010·Diabetes Research and Clinical Practice·R G Ahmed
Jun 25, 2002·Biology of Reproduction·Shannondoah A ColtonStephen M Downs
Sep 1, 1993·Metabolism: Clinical and Experimental·A S PenziasM P Diamond
Dec 31, 1997·American Journal of Reproductive Immunology : AJRI·H N HoY S Yang
Sep 13, 2008·Endocrinology·Masaood GanaiemMahmoud Huleihel
Nov 30, 2000·Upsala Journal of Medical Sciences·U J ErikssonP Wentzel

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