Manipulating conserved heme cavity residues of chlorite dismutase: effect on structure, redox chemistry, and reactivity

Biochemistry
Stefan HofbauerChristian Obinger

Abstract

Chlorite dismutases (Clds) are heme b containing oxidoreductases that convert chlorite to chloride and molecular oxygen. In order to elucidate the role of conserved heme cavity residues in the catalysis of this reaction comprehensive mutational and biochemical analyses of Cld from "Candidatus Nitrospira defluvii" (NdCld) were performed. Particularly, point mutations of the cavity-forming residues R173, K141, W145, W146, and E210 were performed. The effect of manipulation in 12 single and double mutants was probed by UV-vis spectroscopy, spectroelectrochemistry, pre-steady-state and steady-state kinetics, and X-ray crystallography. Resulting biochemical data are discussed with respect to the known crystal structure of wild-type NdCld and the variants R173A and R173K as well as the structures of R173E, W145V, W145F, and the R173Q/W146Y solved in this work. The findings allow a critical analysis of the role of these heme cavity residues in the reaction mechanism of chlorite degradation that is proposed to involve hypohalous acid as transient intermediate and formation of an O═O bond. The distal R173 is shown to be important (but not fully essential) for the reaction with chlorite, and, upon addition of cyanide, it acts as a proton...Continue Reading

References

Nov 1, 1996·Archives of Microbiology·C G van GinkelS W Kengen
Jan 5, 2002·Journal of the American Chemical Society·Gianantonio BattistuzziMarco Sola
Jun 25, 2004·Nucleic Acids Research·Ian W DavisDavid C Richardson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Oct 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Amanda Q LeeJennifer L DuBois
Dec 23, 2008·Cellular and Molecular Life Sciences : CMLS·Y Sugano
Apr 14, 2009·Journal of Molecular Biology·Daniël C de GeusJan Pieter Abrahams
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Mar 10, 2010·Archives of Biochemistry and Biophysics·Gianantonio BattistuzziMarco Sola
Mar 26, 2010·Nature·Katharina F EttwigMarc Strous
Apr 2, 2010·Journal of the American Chemical Society·Bennett R StreitJennifer L DuBois
Apr 14, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Brandon R GoblirschCarrie M Wilmot
Mar 1, 2011·Journal of Molecular Biology·Brandon GoblirschJennifer L DuBois
May 26, 2012·Science·P Andrew Karplus, Kay Diederichs
Jun 12, 2012·Biochimica Et Biophysica Acta·Stefan HofbauerChristian Obinger
Dec 18, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Beatrice BlancJennifer L DuBois
Mar 27, 2013·Annual Review of Biochemistry·David J VinyardG Charles Dismukes
Jun 26, 2013·Acta Crystallographica. Section D, Biological Crystallography·K Diederichs, P A Karplus

❮ Previous
Next ❯

Citations

Jan 21, 2015·Archives of Biochemistry and Biophysics·Stefan HofbauerPaul G Furtmüller
Feb 26, 2015·Archives of Biochemistry and Biophysics·Harry A Dailey, Svetlana Gerdes
Mar 10, 2015·Archives of Biochemistry and Biophysics·Irene SchaffnerChristian Obinger
Mar 18, 2015·Archives of Biochemistry and Biophysics·Arianna I Celis, Jennifer L DuBois
Feb 10, 2016·Bioscience Reports·Stefan HofbauerChristian Obinger
Mar 4, 2015·Molecular Microbiology·Irene SchaffnerChristian Obinger
Oct 29, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Durga MahorPeter-Leon Hagedoorn
Sep 7, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Stefan HofbauerPaul G Furtmüller
Oct 28, 2017·Journal of Inorganic Biochemistry·Marzia BelleiGianantonio Battistuzzi
Feb 7, 2018·Biochemistry·Zachary GeeraertsGudrun S Lukat-Rodgers
Sep 3, 2021·Biological Reviews of the Cambridge Philosophical Society·Luděk KořenýJulius Lukeš

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
PCR

Software Mentioned

MOLREP
XDS
XSCALE
COOT
Clds
Proteum2
MOLPROBITY
PHENIX suite

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.