Manipulating signal delivery - plasma-membrane ERK activation in aPKC-dependent migration

Journal of Cell Science
Katrina BoeckelerPeter J Parker

Abstract

Members of the PKC superfamily have been implicated in various migratory models and in particular in spatially restricted processes. However, defining the precise local events that underlie the PKC-dependent processes is constrained by the unspecific nature of interventions. Here we address this problem in relation to atypical PKC (aPKC) action, which in conjunction with the exocyst complex controls the polarised delivery of promigratory signals. A drug-dependent recruitment approach was employed to manipulate the local recruitment of signals to the leading edge of migrating cells, under conditions where the aPKC-exocyst control is globally abrogated. We found that activation of ERK but not JNK at focal adhesions recovers the majority of the migratory loss attributed to ERK action, demonstrating a necessary role for active plasma membrane ERK in the downstream signalling of aPKC-dependent migration. The data further show that restored focal adhesion dynamics are a contributing mechanism through which localized ERK activity influences this aPKC-exocyst-dependent migration.

References

Apr 21, 1997·The Journal of Cell Biology·R L KlemkeD A Cheresh
Jul 11, 1998·The Journal of Biological Chemistry·M F FavataJ M Trzaskos
May 16, 2002·The Journal of Biological Chemistry·Geraldine Pawlak, David M Helfman
Mar 1, 2003·Nature·Sandrine Etienne-Manneville, Alan Hall
Jul 24, 2003·The Journal of Cell Biology·Jill K Slack-DavisAndrew D Catling
Oct 25, 2003·Journal of Cell Science·Ronen Zaidel-BarBenjamin Geiger
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Jan 27, 2004·Nature Cell Biology·Donna J WebbAlan F Horwitz
May 8, 2004·Trends in Cell Biology·Neil O Carragher, Margaret C Frame
Sep 28, 2004·Nature Cell Biology·Santos J FrancoAnna Huttenlocher
Sep 15, 2005·The Journal of Cell Biology·Sandrine Etienne-MannevilleAlan Hall
Oct 29, 2005·Journal of Cell Science·Donna J WebbA Rick Horwitz
Mar 10, 2006·Journal of Cell Science·Atsushi Suzuki, Shigeo Ohno
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·N FiliB Larijani
Jul 5, 2007·Developmental Cell·Takashi Nishimura, Kozo Kaibuchi
Nov 21, 2007·Seminars in Cancer Biology·John G LockStaffan Strömblad
Dec 18, 2007·Current Opinion in Cell Biology·Joshua A BroussardIrina Kaverina

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Citations

Oct 15, 2011·Protoplasma·Jianglan Liu, Wei Guo
Jul 2, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qin WangChristopher A McCulloch
Feb 14, 2013·Biochimica Et Biophysica Acta·Nick PeelPeter J Parker
Nov 4, 2015·Stem Cell Reports·In Kyoung MahFrancesca V Mariani
Jan 18, 2011·The Biochemical Journal·Maud Hertzog, Philippe Chavrier
May 21, 2015·Science Signaling·Michelle C MendozaGaudenz Danuser
Feb 27, 2015·Chemical Communications : Chem Comm·Kalyn A BrownAlexander Deiters
May 30, 2019·The Journal of Biological Chemistry·Shiela C SamsonMichelle C Mendoza
Apr 27, 2021·Experimental Cell Research·Srikanth Swamy Swaroop BGanesh Venkatraman
Apr 4, 2013·Journal of Cell Science·Simone RadtkePeter J Parker

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