Manipulating the glycosylation pathway in bacterial and lower eukaryotes for production of therapeutic proteins

Current Opinion in Biotechnology
Diana Chinyere Anyaogu, Uffe Hasbro Mortensen

Abstract

The medical use of pharmaceutical proteins is rapidly increasing and cheap, fast and efficient production is therefore attractive. Microbial production hosts are promising candidates for development and production of pharmaceutical proteins. However, as most therapeutic proteins are secreted proteins, they are frequently N-glycosylated. This hampers production in microbes as these hosts glycosylate proteins differently. The resulting products may therefore be immunogenic, unstable and show reduced efficacy. Recently, successful glycoengineering of microbes has demonstrated that it is possible to produce proteins with humanlike glycan structures setting the stage for production of pharmaceutical proteins in bacteria, yeasts and algae.

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Nov 12, 2016·World Journal of Microbiology & Biotechnology·Amjad Hayat KhanAzam Rahimpour
Mar 10, 2017·Trends in Biotechnology·Timo Johannssen, Bernd Lepenies
Dec 22, 2017·Critical Reviews in Biotechnology·Justin Bryan Goh, Say Kong Ng
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Jun 27, 2021·Metabolic Engineering·Diana Chinyere AnyaoguUffe Hasbro Mortensen
Aug 17, 2016·Bioconjugate Chemistry·Zhigang WuPeng George Wang

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