Manipulating the inter pillar gap in pillar array ultra-thin layer planar chromatography platforms

The Analyst
Nichole A CraneMichael J Sepaniak

Abstract

An advantage of separation platforms based on deterministic micro- and nano-fabrications, relative to traditional systems based on packed beds of particles, is the exquisite control of all morphological parameters. For example, with planar platforms based on lithographically-prepared pillar arrays, the size, shape, height, geometric arrangement, and inter pillar gaps can be independently adjusted. Since the inter pillar gap is expected to be important in determining resistance to mass transfer in the mobile phase as well as the flow rate, which influences the mass transfer effect and axial diffusion, we herein study the effect of reducing inter pillar gaps on capillary action-based flow and band dispersion. Atomic layer deposition is used to narrow the gap between the pillars for photo-lithographically defined pillar arrays. The plate height of gap-adjusted arrays is modeled based on predicted and observed flow rates. A reduction in the flow rate with smaller gaps hinders the efficiency in the modeled case and is correlated with actual separations. A conclusion is drawn that simultaneously reducing both the gap and the pillar diameter is the best approach in terms of improving the chromatographic efficiency.

References

Sep 10, 2004·Journal of Separation Science·Piotr GzilGert Desmet
Nov 22, 2007·Lab on a Chip·Wim De MalscheHan Gardeniers
Jun 19, 2008·Analytical Chemistry·Wim De MalscheGert Desmet
Apr 2, 2010·Lab on a Chip·Nickolay V LavrikMichael J Sepaniak
May 1, 1996·Analytical Chemistry·J P ChervetJ P Salzmann
Dec 24, 2011·The Analyst·Lisa C TaylorMichael J Sepaniak
Feb 22, 2012·Journal of Chromatography. a·Wim De MalscheFrederic Lynen
Jul 13, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Trong Thi MaiW K Choi
Nov 20, 2012·Analytical Chemistry·Gert Desmet, Sebastiaan Eeltink
Nov 16, 2013·Analytical Chemistry·Teresa B KirchnerMichael J Sepaniak
Sep 24, 2014·ACS Applied Materials & Interfaces·Jennifer J CharltonMichael J Sepaniak
Nov 5, 2014·Analytical Chemistry·Ryan A WallaceMichael J Sepaniak
Apr 11, 2015·The Analyst·Teresa B KirchnerMichael J Sepaniak
Jun 5, 2015·Analytical Chemistry·Jennifer J CharltonMichael J Sepaniak

❮ Previous
Next ❯

Citations

Aug 12, 2016·Analytical Chemistry·Danielle R LincolnMichael J Sepaniak
May 16, 2017·Electrophoresis·Rachel B StrickhouserMichael J Sepaniak
Jan 27, 2019·Journal of Chromatography. a·Joseph A KraaiAlan X Wang
Jul 10, 2017·Journal of Chromatography. a·Shunta FutagamiWim De Malsche

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved