Manipulating the Mitochondrial Genome To Enhance Cattle Embryo Development

G3 : Genes - Genomes - Genetics
Kanokwan Srirattana, Justin C St John

Abstract

The mixing of mitochondrial DNA (mtDNA) from the donor cell and the recipient oocyte in embryos and offspring derived from somatic cell nuclear transfer (SCNT) compromises genetic integrity and affects embryo development. We set out to generate SCNT embryos that inherited their mtDNA from the recipient oocyte only, as is the case following natural conception. While SCNT blastocysts produced from Holstein (Bos taurus) fibroblasts depleted of their mtDNA, and oocytes derived from Angus (Bos taurus) cattle possessed oocyte mtDNA only, the coexistence of donor cell and oocyte mtDNA resulted in blastocysts derived from nondepleted cells. Moreover, the use of the reprogramming agent, Trichostatin A (TSA), further improved the development of embryos derived from depleted cells. RNA-seq analysis highlighted 35 differentially expressed genes from the comparison between blastocysts generated from nondepleted cells and blastocysts from depleted cells, both in the presence of TSA. The only differences between these two sets of embryos were the presence of donor cell mtDNA, and a significantly higher mtDNA copy number for embryos derived from nondepleted cells. Furthermore, the use of TSA on embryos derived from depleted cells positively mo...Continue Reading

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Datasets Mentioned

BETA
SRP078663
GSE97912

Methods Mentioned

BETA
histone acetylation
PCR
Electrophoresis
RNA-Seq
Feature Extraction
Fluorescence

Software Mentioned

Ingenuity Pathway Analysis ( IPA )
Agilent Genomic Workbench
Feature Extraction
featureCounts
EdgeR
SAS
Statistical Analysis System
LightScanner HRM
GraphPad Prism
GraphPad

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