Manipulating the pyruvate dehydrogenase bypass of a multi-vitamin auxotrophic yeast Torulopsis glabrata enhanced pyruvate production

Letters in Applied Microbiology
L-M LiuJ Chen

Abstract

To investigate the relationship between the activity of pyruvate dehydrogenase (PDH) bypass and the production of pyruvate of a multi-vitamin auxotrophic yeast Torulopsis glabrata. Torulopsis glabrata CCTCC M202019, a multi-vitamin auxotrophic yeast that requires acetate for complete growth on glucose minimum medium, was selected after nitrosoguanidine mutagenesis of the parent strain T. glabrata WSH-IP303 screened in previous study [Li et al. (2001) Appl. Microbiol. Biotechnol. 55, 680-685]. Strain CCTCC M202019 produced 21% higher pyruvate than the parent strain and was genetically stable in flask cultures. The activities of the pyruvate metabolism-related enzymes in parent and mutant strains were measured. Compared with the parent strain, the activity of pyruvate decarboxylase (PDC) of the mutant strain CCTCC M202019 decreased by roughly 40%, while the activity of acetyl-CoA synthetase (ACS) of the mutant increased by 103.5 or 57.4%, respectively, in the presence or absence of acetate. Pyruvate production by the mutant strain CCTCC M202019 reached 68.7 g l(-1) at 62 h (yield on glucose of 0.651 g g(-1)) in a 7-l jar fermentor. The increased pyruvate yield in T. glabrata CCTCC M202019 was due to a balanced manipulation of the...Continue Reading

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Citations

Jan 13, 2006·Applied Microbiology and Biotechnology·Liming LiuJian Chen
Oct 4, 2011·Applied Microbiology and Biotechnology·Christina OttoGerold Barth
Feb 25, 2014·Applied Microbiology and Biotechnology·Xiaoxia YuLiming Liu
Jul 6, 2010·Plasmid·Jingwen ZhouJian Chen
Dec 6, 2014·Metabolic Engineering·Shubo LiJian Chen
Apr 10, 2008·Journal of Bioscience and Bioengineering·Ping XuCuiqing Ma
Dec 13, 2006·Biotechnology and Bioengineering·Liming LiuJian Chen
Oct 15, 2013·Biotechnology Progress·Songxin YangJian Chen
Feb 25, 2015·Metabolic Engineering·Xiulai ChenLiming Liu
Sep 9, 2015·Frontiers in Microbiology·Jing WuLiming Liu
May 28, 2013·Metabolic Engineering·Xiulai ChenJian Chen
Sep 25, 2016·Applied and Environmental Microbiology·Dongni YanJian Chen
Feb 3, 2005·Yeast
Jun 13, 2020·Biotechnology and Bioengineering·Xiulai ChenLiming Liu
Mar 28, 2017·Applied Microbiology and Biotechnology·Zhengshan LuoJian Chen
Mar 20, 2019·Folia Microbiologica·Krzysztof CybulskiAnita Rywińska
Oct 19, 2017·Biotechnology and Bioengineering·Zhengshan LuoJian Chen

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