Manipulation of amino acid composition in soybean seeds by the combination of deregulated tryptophan biosynthesis and storage protein deficiency.

Plant Cell Reports
Yoichi KitaMasao Ishimoto

Abstract

The ability of genetic manipulation to yield greatly increased concentrations of free amino acids (FAAs) in seeds of soybean was evaluated by introduction of a feedback-insensitive mutant enzyme of tryptophan (Trp) biosynthesis into two transformation-competent breeding lines deficient in major seed storage proteins. The storage protein-deficient lines exhibited increased accumulation of certain other seed proteins as well as of FAAs including arginine (Arg) and asparagine in mature seeds. Introduction of the gene for a feedback-insensitive mutant of an alpha subunit of rice anthranilate synthase (OASA1D) into the two high-FAA breeding lines by particle bombardment resulted in a >10-fold increase in the level of free Trp in mature seeds compared with that in nontransgenic seeds. The amount of free Trp in these transgenic seeds was similar to that in OASA1D transgenic seeds of the wild-type cultivar Jack. The composition of total amino acids in seeds of the high-FAA breeding lines remained largely unaffected by the expression of OASA1D with the exception of an increase in the total Trp content. Our results therefore indicate that the extra nitrogen resource originating from storage protein deficiency was used exclusively for the...Continue Reading

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Citations

Apr 12, 2016·Frontiers in Plant Science·Sudhakar PanduranganFrédéric Marsolais
Jun 21, 2017·International Journal of Molecular Sciences·Guoping WangGad Galili
Apr 23, 2015·Physiologia Plantarum·Sudhakar PanduranganFrédéric Marsolais
Oct 3, 2018·International Journal of Tryptophan Research : IJTR·Mendel Friedman
Oct 11, 2020·Metabolomics : Official Journal of the Metabolomic Society·Mohamed Bedair, Kevin C Glenn
Mar 19, 2021·Frontiers in Plant Science·Samantha Vivia TheMechthild Tegeder

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