Manipulation of baculovirus vectors

Molecular Biotechnology
C L MerringtonR D Possee

Abstract

Baculovirus expression vectors provide an excellent system for the synthesis of recombinant proteins in insect cells. This article presents sufficient background information to allow the nonspecialist to understand the basic principles of the technology and the development of baculovirus expression vectors. A summary of the most commonly used plasmids and viruses is presented. Detailed techniques are described to enable recombinant baculoviruses to be constructed. These methods include the protocols required for propagating insect cells in culture and their subsequent infection with viruses.

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Citations

Oct 18, 2002·Journal of Invertebrate Pathology·Helen J MatthewsJohn P Edwards
Oct 18, 2002·Journal of Invertebrate Pathology·Alina V GoldbergAlicia Sciocco de Cap
Oct 18, 2002·Journal of Invertebrate Pathology·Elizabeth M BrooksTerry N Hanzlik
Jun 6, 2003·Journal of Invertebrate Pathology·James J BecnelAlexandra M Shapiro
Jul 5, 2003·Virology·Sally WormleatonDoreen Winstanley
Sep 1, 2000·Advanced Drug Delivery Reviews·F NilssonD Neri
Jul 9, 2011·PLoS Computational Biology·Wopke van der WerfMark P Zwart
Nov 23, 2013·Biotechnology Progress·A Contreras-GómezY Chisti
Dec 23, 1999·Current Biology : CB·G Gibson
Oct 20, 2005·Journal of Biomolecular Screening·Wayne R LeifertEdward J McMurchie
Feb 5, 2008·Molecular Biology Reports·Jun-Qing GeChuan-Xi Zhang
Oct 11, 2011·Biotechnology Advances·Jean-Christophe DrugmandSpiros N Agathos

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