Manipulation of catalase levels produces altered photosynthesis in transgenic tobacco plants

Plant Physiology
L F BrissonE A Havir

Abstract

Constructs containing the cDNAs encoding the primary leaf catalase in Nicotiana or subunit 1 of cottonseed (Gossypium hirsutum) catalase were introduced in the sense and antisense orientation into the Nicotiana tabacum genome. The N. tabacum leaf cDNA specifically overexpressed CAT-1, the high catalatic [corrected] form, activity. Antisense constructs reduced leaf catalase specific activities from 0.20 to 0.75 times those of wild type (WT), and overexpression constructs increased catalase specific activities from 1.25 to more than 2.0 times those of WT. The NADH-hydroxypyruvate reductase specific activity in transgenic plants was similar to that in WT. The effect of antisense constructs on photorespiration was studied in transgenic plants by measuring the CO2 compensation point (gamma) at a leaf temperature of 38 degrees C. A significant linear increase was observed in gamma with decreasing catalase (at 50% lower catalase activity gamma increased 39%). There was a significant temperature-dependent linear decrease in gamma in transgenic leaves with elevated catalase compared with WT leaves (at 50% higher catalase gamma decreased 17%). At 29 degrees C, gamma also decreased with increasing catalase in transgenic leaves compared wi...Continue Reading

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Citations

Oct 16, 2007·Biotechnology Letters·Prosenjit MondalSurendra Ch Sabat
Apr 29, 2000·Trends in Plant Science·A D HansonY Shachar-Hill
Sep 30, 2010·Journal of Experimental Botany·Amna MhamdiGraham Noctor
Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·G NoctorC H Foyer
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May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Andrew D Hanson, Sanja Roje
Mar 11, 2010·Critical Reviews in Biotechnology·Parvaiz AhmadSatyawati Sharma
Mar 13, 2016·Journal of Experimental Botany·Stefan TimmHermann Bauwe
Jun 16, 2015·Journal of Plant Physiology·José Ramón Acosta-MotosJosé Antonio Hernández
Aug 12, 2000·Journal of Experimental Botany·J M Dunwell
Jan 1, 2009·The Arabidopsis Book·Navneet KaurJianping Hu
Jan 17, 2020·Antioxidants·Grisaly GarcíaJosé Antonio Hernández
Nov 30, 2019·Plant Science : an International Journal of Experimental Plant Biology·B H FengG F Fu

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