Manipulation of cytokinin level in the ergot fungus Claviceps purpurea emphasizes its contribution to virulence

Current Genetics
Sabine KindPetr Galuszka

Abstract

Pathogen-derived cytokinins (CKs) have been recognized as important virulence factor in several host-pathogen interactions and it was demonstrated multiple times that phytopathogenic fungi form CKs via the tRNA degradation pathway. In contrast to previous studies, the focus of this study is on the second step of CK formation and CK degradation to improve our understanding of the biosynthesis in fungi on the one hand, and to understand CK contribution to the infection process of Claviceps purpurea on the other hand. The ergot fungus Claviceps purpurea is a biotrophic phytopathogen with a broad host range including economically important crops causing harvest intoxication upon infection. Its infection process is restricted to unfertilized ovaries without causing macroscopic defense symptoms. Thus, sophisticated host manipulation strategies are implicated. The cytokinin (CK) plant hormones are known to regulate diverse plant cell processes, and several plant pathogens alter CK levels during infection. C. purpurea synthesizes CKs via two mechanisms, and fungus-derived CKs influence the host-pathogen interaction but not fungus itself. CK deficiency in fungi with impact on virulence has only been achieved to date by deletion of a tRN...Continue Reading

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Citations

Nov 30, 2018·Plant, Cell & Environment·Anne CortlevenThomas Schmülling
Mar 7, 2020·Frontiers in Plant Science·Saqib Saleem AkhtarThomas Roitsch
Mar 20, 2020·Planta·Irina E DoduevaLudmila L Lutova

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Methods Mentioned

BETA
PCR
RNA-seq
Protein Assay
dissection
ribosylation
gene knockouts
transgenic
gene knockout

Software Mentioned

DNA Star
BLAST
LabSolutions
FeatureCounts
Tophat
BLASTp
DESeq2
Primer Express

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