Manipulation of environmental oxygen modifies reactive oxygen and nitrogen species generation during myogenesis

Redox Biology
Rachel McCormickAphrodite Vasilaki

Abstract

Regulated changes in reactive oxygen and nitrogen species (RONS) activities are important in maintaining the normal sequence and development of myogenesis. Both excessive formation and reduction in RONS have been shown to affect muscle differentiation in a negative way. Cultured cells are typically grown in 20% O2 but this is not an appropriate physiological concentration for a number of cell types, including skeletal muscle. The aim was to examine the generation of RONS in cultured skeletal muscle cells under a physiological oxygen concentration condition (6% O2) and determine the effect on muscle myogenesis. Primary mouse satellite cells were grown in 20% or 6% O2 environments and RONS activity was measured at different stages of myogenesis by real-time fluorescent microscopy using fluorescent probes with different specificities i.e. dihydroethidium (DHE), 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate (DAF-FM DA) and 5-(and-6)-chloromethyl-2',7' -dichlorodihydrofluorescein diacetate (CM-DCFH-DA). Data demonstrate that satellite cell proliferation increased when cells were grown in 6% O2 compared with 20% O2. Myoblasts grown in 20% O2 showed an increase in DCF fluorescence and DHE oxidation compared with myoblasts ...Continue Reading

References

Sep 1, 1976·The American Journal of Anatomy·E Schultz
Dec 8, 1994·Nature·L KobzikJ S Stamler
Sep 1, 2001·Cellular and Molecular Life Sciences : CMLS·M V ChakravarthyF W Booth
Dec 19, 2001·Microscopy Research and Technique·C L Murrant, M B Reid
Jan 13, 2004·Physiological Reviews·Sophie B P Chargé, Michael A Rudnicki
Jun 21, 2005·Annals of the New York Academy of Sciences·Marie Csete
Aug 9, 2005·Free Radical Biology & Medicine·Francis McArdleMalcolm J Jackson
Sep 12, 2006·Free Radical Biology & Medicine·Sukkyoo LeeMarie E Csete
Jan 24, 2007·Cell Biology International·Jason M HansenMarie Csete
Dec 31, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Youngmok C JangHolly Van Remmen
Apr 1, 2007·Proteomics. Clinical Applications·Aphrodite VasilakiMalcolm J Jackson
Sep 1, 2012·PloS One·Stephanie DuguezTerence A Partridge
May 24, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jesus PalomeroMalcolm J Jackson

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Citations

Apr 22, 2017·Journal of the American Society of Nephrology : JASN·Jun-Yi ZhuZhe Han
Nov 17, 2016·Oxidative Medicine and Cellular Longevity·Adriano César Carneiro LoureiroDenise P Carvalho

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Methods Mentioned

BETA
fluorescence microscopy
FCS
fluorescence imaging microscopy

Software Mentioned

EZC1

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