PMID: 9448037Feb 3, 1998Paper

Manipulation of Th1/Th2 balance in vivo by adoptive transfer of antigen-specific Th1 or Th2 cells

Journal of Immunological Methods
A OhtaT Nishimura

Abstract

We have investigated the possibility that the Th1/Th2 balance in vivo may be modulated by adoptive transfer of Th1 or Th2 cells induced in vitro. Thl cells were induced from I-Ad-binding OVA323-339-specific T-cell receptor-transgenic (TCR-Tg) mouse spleen cells by culturing with OVA323-339 peptide and antigen presenting cells (APC) in the presence of IL-2, IL-12 and anti-IL-4 mAb. Th2 cells were induced from TCR-Tg mouse spleen cells by culturing with IL-2, IL-4 and anti-IL-12 mAb in addition to OVA323-339 plus APC. Immunomodulating activities of both Th1 and Th2 cells were determined by their effect on delayed type hypersensitivity (DTH) responses or cytokine production. No significant DTH responses (footpad swelling) were observed in untreated BALB/c mice following a single injection of OVA323-339-pulsed syngeneic spleen cells. However, adoptive transfer of Th1 cells into BALB/c mice induced strong dose dependent DTH responses in response to I-Ad-bound OVA323-339 but not unrelated peptide. In contrast, only slight DTH responses were detected in BALB/c mice transferred with Th2 cells. In parallel with the DTH responses, increased levels of serum IFN-gamma were demonstrated in mice adoptively transferred with Th1, while no sign...Continue Reading

References

Jan 1, 1992·Annual Review of Immunology·R MartinD E McFarlin
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C S HsiehK M Murphy
Oct 1, 1991·Immunology Today·P Scott, S H Kaufmann
Aug 1, 1990·The Journal of Experimental Medicine·A JardimR W Olafson
Nov 1, 1994·The Journal of Experimental Medicine·M K RackeM Röcken
Sep 1, 1995·The Journal of Experimental Medicine·J A GollobJ Ritz
Jan 1, 1993·The Journal of Experimental Medicine·J L BaronC A Janeway
Mar 1, 1995·The Journal of Experimental Medicine·A H Shankar, R G Titus
Dec 1, 1993·Current Opinion in Immunology·A O'Garra, K Murphy
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·A K SimonJ Sieper
May 1, 1993·The Journal of Experimental Medicine·F P HeinzelM K Gately
Apr 1, 1996·The Journal of Experimental Medicine·L BianconeI Stamenkovic
Apr 1, 1996·International Immunology·Y TanakaT Nishimura
Oct 31, 1996·Annals of the New York Academy of Sciences·T NishimuraS Habu

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Citations

Jul 27, 2007·Clinical & Experimental Metastasis·Hiroshi YokouchiTakashi Nishimura
Sep 8, 1999·The Journal of Experimental Medicine·T NishimuraA Ohta
Mar 18, 2003·Molecular and Cellular Biology·Martina SeiffertLarry R Rohrschneider
Jan 24, 2007·Biochimica Et Biophysica Acta·Yoshinori Mine, Marie Yang
Jan 1, 1997·Springer Seminars in Immunopathology·S J Czinn, J G Nedrud
Dec 22, 1999·The Proceedings of the Nutrition Society·B Lesourd, L Mazari
Mar 21, 2008·European Journal of Heart Failure·Girolamo CatapanoRaffaele Antonelli Incalzi
May 27, 1999·The Proceedings of the Nutrition Society·B Lesourd
Nov 30, 2012·Transplant International : Official Journal of the European Society for Organ Transplantation·Timm HeinbokelStefan G Tullius
Feb 19, 2016·Proceedings. Biological Sciences·Christopher BeirneAndrew Young
Feb 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael T Hopfenspirger, Devendra K Agrawal
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S Kumar, R L Tarleton
Jun 12, 2008·Journal of Agricultural and Food Chemistry·Yoshinori Mine, Marie Yang
Jun 8, 2013·Bioconjugate Chemistry·Chunxia ZouChristopher W Cairo

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