Abstract
Recent studies suggested that modification of the membrane contact site of vitamin K-dependent proteins may enhance the membrane affinity and function of members of this protein family. The properties of a factor VII mutant, factor VII-Q10E32, relative to wild-type factor VII (VII, containing P10K32), have been compared. Membrane affinity of VII-Q10E32 was about 20-fold higher than that of wild-type factor VII. The rate of autoactivation VII-Q10E32 with soluble tissue factor was 100-fold faster than wild-type VII and its rate of activation by factor Xa was 30 times greater than that of wild-type factor VII. When combined with soluble tissue factor and phospholipid, activated factor VII-Q10E32 displayed increased activation of factor X. Its coagulant activity was enhanced in all types of plasma and with all sources of tissue factor tested. This difference in activity (maximum 50-fold) was greatest when coagulation conditions were minimal, such as limiting levels of tissue factor and/or phospholipid. Because of its enhanced activity, factor VII-Q10E32 and its derivatives may provide important reagents for research and may be more effective in treatment of bleeding and/or clotting disorders.
References
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B Osterud, S I Rapaport
May 30, 1978·Biochemistry·G L NelsestuenR G Di Scipio
Sep 20, 1977·Biochemistry·G L Nelsestuen, T K Lim
May 7, 1976·Analytical Biochemistry·M M Bradford
Nov 4, 1975·Biochemistry·W Kisiel, E W Davie
Mar 19, 1992·The New England Journal of Medicine·B Furie, B C Furie
Nov 12, 1991·Biochemistry·T NakagakiW Kisiel
Apr 10, 1990·Biochemistry·L C PetersenD Foster
Nov 28, 1989·Biochemistry·A H PedersenL C Petersen
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L V Rao, S I Rapaport
Dec 1, 1986·Thrombosis Research·P P NawrothD M Stern
Oct 1, 1971·Biochemical and Biophysical Research Communications·G L Nelsestuen, J W Suttie
Jan 1, 1969·Biochemistry·C Huang
Jan 9, 1996·Biochemistry·L C PetersenW Kisiel
Jan 1, 1995·Advances in Experimental Medicine and Biology·S GlazerJ F Falch
Jan 1, 1996·Haemostasis·U Hedner
Feb 1, 1997·Journal of Vascular Surgery·B ArnljotsU Hedner
Apr 29, 1997·Biochemistry·J F McDonaldG L Nelsestuen
Jan 24, 1998·Biochemistry·L ShenG L Nelsestuen
Feb 4, 2005·Journal of Health Politics, Policy and Law·Mark Schlesinger
Citations
Nov 30, 2006·European Biophysics Journal : EBJ·Olivier Taboureau, Ole Hvilsted Olsen
Feb 14, 2002·Trends in Cardiovascular Medicine·Charles Eigenbrot, Daniel Kirchhofer
Jan 20, 2000·Trends in Cardiovascular Medicine·G L Nelsestuen
Mar 13, 2012·Thrombosis Research·Egon Persson
May 13, 2011·Thrombosis Research·Laura D GrayAlisa S Wolberg
Jul 25, 2012·Biophysical Journal·Y Zenmei OhkuboEmad Tajkhorshid
Mar 8, 2005·Blood Reviews·Evangelos TerposAmin Rahemtulla
Nov 13, 1998·The Journal of Biological Chemistry·L ShenG L Nelsestuen
Nov 26, 2002·Blood·Yong-Hui SunBjörn Dahlbäck
Dec 31, 2002·The Journal of Biological Chemistry·Stephen B HarveyGary L Nelsestuen
Aug 23, 2001·The Journal of Biological Chemistry·G L NelsestuenW Kisiel
Sep 28, 2002·Blood·Suely M RezendeRachel E Simmonds
May 28, 2021·Protein Expression and Purification·Abhinav LuthraWilfried A M Bakker
Jul 25, 2013·Biochemistry·Narjes TavoosiJames H Morrissey