PMID: 7544912Sep 1, 1995Paper

Mannan-binding protein forms complexes with alpha-2-macroglobulin. A protein model for the interaction

Scandinavian Journal of Immunology
P StorgaardS E Svehag

Abstract

We report that alpha-2-macroglobulin (alpha 2M) can form complexes with a high molecular weight porcine mannan-binding protein (pMBP-28). The alpha 2M/pMBP-28 complexes was isolated by PEG-precipitation and affinity chromatography on mannan-Sepharose, protein A-Sepharose and anti-IgM Sepharose. The occurrence of alpha 2M/pMBP-28 complexes was further indicated by crossed immunoelectrophoresis and by use of an anti-alpha 2M affinity column and chelating Sepharose loaded with Zn2+. The eluates from these affinity columns showed alpha 2M subunits (94 and 180 kDa) and pMBP subunits (28kDa) in SDS-PAGE, which reacted with antibodies against alpha 2M and pMBP-28, respectively, in Western blotting. Furthermore, alpha 2M/pMBP-28 complexes were demonstrated by electron microscopy. Fractionation of pMBP-containing D-mannose eluate from mannan-Sepharose on Superose 6 showed two protein peaks which reacted with anti-C1 s antibodies in ELISA, one of about 650-800 kDa, which in addition contained pMBP-28 and anti-alpha 2M reactive material, the other with an M(r) of 100-150 kDa. The latter peak revealed rhomboid molecules (7 x 15 nm) in the electron microscope and a 67 kDa band in SDS-PAGE under reducing conditions. This band was also seen i...Continue Reading

References

Dec 1, 1978·Experimental Cell Research·F Van LeuvenH Van den Berghe
Dec 5, 1975·Biochimica Et Biophysica Acta·M T DebanneJ Dolovich
Mar 1, 1990·Journal of Structural Biology·K E Loesser, C Franzini-Armstrong
Feb 1, 1987·Scandinavian Journal of Clinical and Laboratory Investigation·C Munck PetersenJ Gliemann
Apr 1, 1988·European Journal of Clinical Investigation·C M PetersenJ Ingerslev
Apr 30, 1985·Biochemical and Biophysical Research Communications·S R FeldmanS V Pizzo
Jan 1, 1988·Archives of Biochemistry and Biophysics·S OkaI Yamashina
Jul 26, 1972·Nature: New Biology·S E SvehagB Bloth
Apr 1, 1968·The Journal of Experimental Medicine·B BlothS E Svehag
Sep 6, 1984·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M G ColombC L Villiers
Jan 1, 1984·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·I BrandslundS E Svehag
Feb 1, 1984·Scandinavian Journal of Clinical and Laboratory Investigation·J SelmerI Clemmensen
Sep 1, 1983·Journal of Biochemistry·N KawasakiI Yamashina
Jul 31, 1980·Biochemical and Biophysical Research Communications·Y KozutsumiI Yamashina
Feb 1, 1994·Scandinavian Journal of Immunology·P HoltJ C Jensenius
Apr 1, 1993·Glycobiology·U HolmskovJ C Jensenius

Citations

Sep 7, 2002·Journal of Immunological Methods·Saskia KuipersHans van Dijk
Mar 16, 2000·Developmental and Comparative Immunology·S B Laursen, O L Nielsen
Mar 21, 2001·Molecular Immunology·S V PetersenJ C Jensenius
Jan 13, 2001·Current Opinion in Immunology·M GadjevaJ C Jensenius
Apr 2, 1998·Current Opinion in Immunology·M MatsushitaT Fujita
Oct 6, 2000·APMIS. Supplementum·U Holmskov
May 12, 2007·Veterinary Immunology and Immunopathology·Lise R PhaneufPatricia V Turner
Aug 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T Vorup-JensenJ C Jensenius
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sunita GulatiSanjay Ram

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