PMID: 7544912Sep 1, 1995Paper

Mannan-binding protein forms complexes with alpha-2-macroglobulin. A protein model for the interaction

Scandinavian Journal of Immunology
P StorgaardS E Svehag

Abstract

We report that alpha-2-macroglobulin (alpha 2M) can form complexes with a high molecular weight porcine mannan-binding protein (pMBP-28). The alpha 2M/pMBP-28 complexes was isolated by PEG-precipitation and affinity chromatography on mannan-Sepharose, protein A-Sepharose and anti-IgM Sepharose. The occurrence of alpha 2M/pMBP-28 complexes was further indicated by crossed immunoelectrophoresis and by use of an anti-alpha 2M affinity column and chelating Sepharose loaded with Zn2+. The eluates from these affinity columns showed alpha 2M subunits (94 and 180 kDa) and pMBP subunits (28kDa) in SDS-PAGE, which reacted with antibodies against alpha 2M and pMBP-28, respectively, in Western blotting. Furthermore, alpha 2M/pMBP-28 complexes were demonstrated by electron microscopy. Fractionation of pMBP-containing D-mannose eluate from mannan-Sepharose on Superose 6 showed two protein peaks which reacted with anti-C1 s antibodies in ELISA, one of about 650-800 kDa, which in addition contained pMBP-28 and anti-alpha 2M reactive material, the other with an M(r) of 100-150 kDa. The latter peak revealed rhomboid molecules (7 x 15 nm) in the electron microscope and a 67 kDa band in SDS-PAGE under reducing conditions. This band was also seen i...Continue Reading

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Citations

Sep 7, 2002·Journal of Immunological Methods·Saskia KuipersHans van Dijk
Mar 16, 2000·Developmental and Comparative Immunology·S B Laursen, O L Nielsen
Mar 21, 2001·Molecular Immunology·S V PetersenJ C Jensenius
Jan 13, 2001·Current Opinion in Immunology·M GadjevaJ C Jensenius
Apr 2, 1998·Current Opinion in Immunology·M MatsushitaT Fujita
Oct 6, 2000·APMIS. Supplementum·U L Holmskov
May 12, 2007·Veterinary Immunology and Immunopathology·Lise R PhaneufPatricia V Turner
Aug 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T Vorup-JensenJ C Jensenius
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sunita GulatiSanjay Ram
Jun 5, 2007·Immunobiology·Søren E DegnJens C Jensenius
Apr 20, 2010·Micron : the International Research and Review Journal for Microscopy·M M PaivaT C Araújo-Jorge

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