Mannan Molecular Substructures Control Nanoscale Glucan Exposure in Candida

Cell Reports
Matthew S GrausAaron K Neumann

Abstract

Cell wall mannans of Candida albicans mask β-(1,3)-glucan from recognition by Dectin-1, contributing to innate immune evasion. Glucan exposures are predominantly single receptor-ligand interaction sites of nanoscale dimensions. Candida species vary in basal glucan exposure and molecular complexity of mannans. We used super-resolution fluorescence imaging and a series of protein mannosylation mutants in C. albicans and C. glabrata to investigate the role of specific N-mannan features in regulating the nanoscale geometry of glucan exposure. Decreasing acid labile mannan abundance and α-(1,6)-mannan backbone length correlated most strongly with increased density and nanoscopic size of glucan exposures in C. albicans and C. glabrata, respectively. Additionally, a C. albicans clinical isolate with high glucan exposure produced similarly perturbed N-mannan structures and elevated glucan exposure geometry. Thus, acid labile mannan structure influences the nanoscale features of glucan exposure, impacting the nature of the pathogenic surface that triggers immunoreceptor engagement, aggregation, and signaling.

Citations

Jan 29, 2019·FEMS Yeast Research·Lucia PaulovičováMartin Hrubiško
Jun 20, 2019·Molecular Biology of the Cell·Timothy TraversS Gnanakaran
Feb 13, 2020·Frontiers in Cellular and Infection Microbiology·Fabien Cottier, Rebecca A Hall
Jul 9, 2020·MBio·Delma S ChildersAlistair J P Brown
May 15, 2019·International Journal of Molecular Sciences·Mónica GalochaMiguel C Teixeira
Nov 24, 2019·Nature Communications·Arnab PradhanAlistair J P Brown
Feb 13, 2021·Life·Rohan P Choraghe, Aaron K Neumann
Feb 27, 2021·Applied Microbiology and Biotechnology·Yujie ZhouBiao Ren
Oct 17, 2020·Trends in Microbiology·Arnab PradhanAlistair J P Brown
Apr 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Judith M BainAlistair J P Brown

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