PMID: 11336785May 5, 2001Paper

Mannose 6-phosphate-independent endocytosis of beta-glucuronidase by human fibroblasts. I. Evidence for the existence of a membrane-binding activity

Biochimica Et Biophysica Acta
A González-NoriegaF Masso

Abstract

Prior work has shown that endocytosis of bovine beta-glucuronidase by human fibroblasts can be mediated by the existence of a Man6P-independent receptor for the recapture and targeting to lysosomes. In this study, we have isolated a peptide (IIIb2) from pronase digested bovine beta-glucuronidase that behaved as competitive inhibitor of the endocytosis of bovine beta-glucuronidase by human fibroblasts. This peptide contained a Ser-X-Ser sequence, where X is probably a posttranslational modified Trp. Antibodies raised against this peptide impaired the endocytosis of the bovine but not the human beta-glucuronidase, implying that the new recognition marker for the endocytosis of acid hydrolases might reside in a single discrete stretch of amino acid sequence. On the other hand, bovine beta-glucuronidase has been shown to bind specifically to receptors of human fibroblast membranes. The binding was saturable, divalent cation-dependent and was competitively inhibited by the IIIb2 peptide, but not by mannose 6-phosphate. Results presented suggested an interplay between manganese concentrations, temperature and pH on the dissociation of the beta-glucuronidase-receptor complexes. All together, these data reinforce the presence of two en...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M R NatowiczW S Sly
Jan 1, 1979·Methods in Enzymology·W S Sly, J Grubb
Jan 1, 1986·Annual Review of Biochemistry·K von Figura, A Hasilik
Jan 1, 1986·The Journal of Clinical Investigation·S Kornfeld
Mar 1, 1973·Archives of Biochemistry and Biophysics·P BöhlenS Udenfriend
Apr 14, 1982·Biochemical and Biophysical Research Communications·A WaheedJ G Leroy
Jan 1, 1993·Sub-cellular Biochemistry·P H Weigel
Feb 4, 1997·Biochemistry·E C JimenezB M Olivera

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