Mannose-binding activity of Escherichia coli: a determinant of attachment and ingestion of the bacteria by macrophages.

Infection and Immunity
Z Bar-ShavitD Mirelman

Abstract

Recently, it was suggested that a mannose-specific lectin on the bacterial cell surface is responsible for the recognition by phagocytic cells of certain nonopsonized Escherichia coli strains. In this study we assessed the interaction of two strains of E. coli at different phases of growth with a monolayer of mouse peritoneal macrophages and developed a direct method with [(14)C]mannan to quantitate the bacterial mannose-binding activity. Normal-sized bacteria were obtained from logarithmic and stationary phases of growth. Nonseptated filamentous cells were formed by growing the organisms in the presence of cephalexin or at a restrictive temperature. Attachment to macrophages of all bacterial forms was inhibited by methyl alpha-d-mannoside and mannan but not by other sugars tested. The attachment of stationary phase and filamentous bacteria to macrophages, as well as their mannose-binding activity, was similar, whereas in the exponential-phase bacteria they were markedly reduced. The results show a linear relation between the two parameters (R = 0.98, P < 0.001). The internalization of the filamentous cells attached to macrophages during 45 min of incubation was much less efficient (20%) compared to that of exponential-phase, s...Continue Reading

References

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Citations

Apr 1, 1982·Infection and Immunity·R BrachaD Mirelman
Aug 2, 2003·Molecular Microbiology·Sammia El-LabanyIan C Blomfield
May 1, 1985·Applied and Environmental Microbiology·K HaahtelaT K Korhonen
Jun 1, 1987·Microbiological Reviews·D Mirelman
Mar 1, 1995·Infection and Immunity·D M O'RiordanA H Limper
Jan 1, 1991·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·S Latkovic
Jul 1, 1983·Infection·F J Silverblatt, I Ofek
Jun 1, 1989·FEMS Microbiology Immunology·D M Weir
Nov 1, 1994·Developmental and Comparative Immunology·L BallarinA Sabbadin
Mar 16, 2007·Cellular Microbiology·Göran BergstenCatharina Svanborg
Jun 3, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Yinan WangYang Liu
Jan 1, 1990·International Journal of Immunopharmacology·W K HagmannH J Zweerink
Apr 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·L OhmanO Stendahl

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