Mannose binding lectin gene deficiency increases susceptibility to traumatic brain injury in mice

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
Phoebe H YagerMichael J Whalen

Abstract

Mannose binding lectin (MBL) initiates complement activation and exacerbates tissue damage after systemic ischemia/reperfusion. We tested the hypothesis that MBL activates complement and worsens outcome using two levels of controlled cortical impact (CCI) in mice. After moderate CCI (0.6 mm depth), MBL immunostaining was detected on injured endothelial cells of wild-type (WT) mice and C3d was detected in MBL KO (deficient in MBL A/C) and WT mice, suggesting that MBL is dispensable for terminal complement activation after CCI. Brain neutrophils, edema, blood-brain barrier permeability, gross histopathology, and motor dysfunction were similar in injured MBL KO and WT mice. In mice subjected to mild CCI (0.2 mm), MBL KO mice had almost two-fold increased acute CA3 cell degeneration at 6 h (P<0.01 versus WT). Naive MBL KO mice had decreased brain volume but performed similar to WT mice in two distinct Morris water maze (MWM) paradigms. However, injured MBL KO mice had impaired performance in cued platform trials (P<0.05 versus WT), suggesting a transient nonspatial learning deficit in injured MBL KO mice. The data suggest that MBL deficiency increases susceptibility to CCI through C3-independent mechanisms and that MBL-deficient pa...Continue Reading

References

Sep 1, 1995·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·W StummerR F Keep
Sep 1, 1995·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S L KaczorowskiP M Kochanek
Jan 1, 1993·Brain Research Bulletin·S LehmannJ P Zanetta
Sep 5, 1998·Brain Research. Brain Research Reviews·P F StahelT Kossmann
Jan 10, 2002·Journal of Neurotrauma·B M BellanderM Svensson
Feb 6, 2002·Brain Research Bulletin·Robert T GerlaiHeidi S Phillips
Nov 5, 2002·Experimental Cell Research·Martyn K White, David S Strayer
Jan 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Swen WagnerMichael Loos
Jun 28, 2003·Progress in Molecular and Subcellular Biology·J P Zanetta
Sep 16, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Mario RancanPhilip F Stahel
May 19, 2004·The Journal of Experimental Medicine·Lei ShiR Alan B Ezekowitz
Feb 11, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jack C de la Torre, Gjumrakch Aliev
Apr 27, 2005·Brain Research. Brain Research Reviews·Oliver I SchmidtPhilip F Stahel
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melanie L HartGregory L Stahl
May 11, 2005·Scandinavian Journal of Immunology·M Møller-KristensenM Gadjeva
Jun 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mary C WalshGregory L Stahl
Aug 27, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Patrick M KochanekEdwin K Jackson
Sep 29, 2005·Springer Seminars in Immunopathology·Rikke SørensenJens C Jensenius
Dec 2, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xin-Kang TongEdith Hamel
Dec 22, 2005·Current Opinion in Immunology·Kazue TakahashiR Alan B Ezekowitz
Jan 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mette Møller-KristensenKazue Takahashi
Jul 21, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Giti GarthwaiteJohn Garthwaite
Sep 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ming ZhangMichael C Carroll
Nov 7, 2006·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Naoki OtaniMichael Brenner
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rodney K ChanWilliam G Austen
Nov 30, 2006·Critical Care Medicine·Michelle N GongDavid C Christiani
Feb 1, 2007·Journal of Neurotrauma·Annadora J Bruce-KellerStephen W Scheff
Mar 17, 2007·The Journal of Investigative Dermatology·Mette Møller-KristensenKazue Takahashi
Apr 26, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Zerong YouMichael J Whalen

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Citations

Apr 27, 2012·Journal of Biomedicine & Biotechnology·Mykol LarvieKazue Takahashi
Dec 2, 2011·Journal of Biomedical Science·Miriam D NeherPhilip F Stahel
Feb 10, 2012·Molecular Neurodegeneration·Ping K Yip, Andrea Malaspina
Dec 17, 2015·Journal of Neurochemistry·Zhijian HuangXiaochuan Sun
Nov 26, 2011·Expert Review of Anti-infective Therapy·Kazue Takahashi
Jul 15, 2015·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Jiang XueYa-Qiong Li
Nov 4, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wei YuWei Shen
Sep 24, 2011·Progress in Neurobiology·Adel HelmyPeter J Hutchinson
Jul 17, 2014·Critical Care Medicine·Andrea KleindienstSebastian Brandner
Feb 6, 2009·Journal of Neuroimmunology·Jing LiPremkumar Christadoss
Oct 14, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jugta KhumanMichael J Whalen
May 16, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Benjamin F MillerMichael J Whalen
Nov 27, 2014·Frontiers in Cellular Neuroscience·Franca OrsiniMaria-Grazia De Simoni
May 12, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Daiana De BlasioMaria-Grazia De Simoni
Jan 24, 2018·Journal of Neuroinflammation·Adnan HammadMalik Zaben
Dec 17, 2018·Molecular Neurobiology·Daniel YoungerNamas Chandra
Aug 24, 2019·Frontiers in Cellular Neuroscience·Saef IzzyMichael J Whalen
Mar 20, 2019·Frontiers in Immunology·Sarah M CarpaniniBryan Paul Morgan
Aug 18, 2017·Journal of Neurotrauma·Francesco RoselliMarkus Huber-Lang
Feb 1, 2020·Journal of Neurotrauma·Min ChenDavid C Reutens
Oct 13, 2020·Frontiers in Pediatrics·Cinzia AuritiVincenzo Maria Di Ciommo
Nov 12, 2019·Seminars in Immunology·John D LeeTrent M Woodruff
May 1, 2021·International Journal of Molecular Sciences·Karolina ZiabskaTeresa Zalewska

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Methods Mentioned

BETA
light microscopy

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