PMID: 11604104Oct 18, 2001Paper

Mannosylerythritol lipid, a yeast extracellular glycolipid, shows high binding affinity towards human immunoglobulin G

BMC Biotechnology
Jae Hong ImDai Kitamoto

Abstract

There have been many attempts to develop new materials with stability and high affinity towards immunoglobulins. Some of glycolipids such as gangliosides exhibit a high affinity toward immunoglobulins. However, it is considerably difficult to develop these glycolipids into the practical separation ligand due to their limited amounts. We thus focused our attention on the feasible use of "mannosylerythritol lipid A", a yeast glycolipid biosurfactant, as an alternative ligand for immunoglobulins, and undertook the investigation on the binding between mannosylerythritol lipid A (MEL-A) and human immunoglobulin G (HIgG). In ELISA assay, MEL-A showed nearly the same binding affinity towards HIgG as that of bovine ganglioside GM1. Fab of human IgG was considered to play a more important role than Fc in the binding of HIgG by MEL-A. The bound amount of HIgG increased depending on the attached amount of MEL-A onto poly (2-hydroxyethyl methacrylate) (polyHEMA) beads, whereas the amount of human serum albumin slightly decreased. Binding-amount and -selectivity of HIgG towards MEL-A were influenced by salt species, salt concentration and pH in the buffer solution. The composite of MEL-A and polyHEMA, exhibited a significant binding constan...Continue Reading

References

Nov 3, 1987·Biochemistry·T W Hutchens, J Porath
Apr 21, 1995·Journal of Chromatography. B, Biomedical Applications·A Denizli, E Pişkin
Mar 21, 1998·Current Biology : CB·N M Hooper
Jul 22, 1998·Journal of Immunological Methods·B A HarrisonE Altman
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·J Turková
Jul 9, 1999·Journal of Molecular Recognition : JMR·S V Evans, C Roger MacKenzie
Nov 26, 1999·Applied Microbiology and Biotechnology·H S KimY Tani
Jan 25, 2000·Journal of Clinical Laboratory Analysis·S J Alié-DaramJ M Dugoujon
Mar 1, 2000·Journal of Neuroimmunology·H J Willison, G M O'Hanlon
Mar 17, 2000·Immunological Reviews·T Feizi
Jun 16, 2000·Applied Microbiology and Biotechnology·I M BanatS S Cameotra
Nov 6, 2004·Brain Research. Developmental Brain Research·Jürgen KopitzJosé Abad-Rodríguez

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Citations

May 15, 2003·Journal of Biomedical Materials Research. Part a·Jae Hong ImDai Kitamoto
Feb 14, 2006·The Journal of Antimicrobial Chemotherapy·Lígia RodriguesRosário Oliveira
Aug 22, 2008·Journal of Industrial Microbiology & Biotechnology·Joseph Irudayaraj ArutchelviMukesh Doble

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