Mannosylinositol phosphorylceramides and ergosterol coodinately maintain cell wall integrity in the yeast Saccharomyces cerevisiae

The FEBS Journal
Seiya Tanaka, Motohiro Tani

Abstract

In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of their normal composition causes several cellular dysfunctions. Previously we found that loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC) causes a defect in cell wall integrity (CWI). In this study, we screened for multicopy suppressor genes that rescue the defect in CWI in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis. In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in CWI. The repression of ERG9 and/or the deletion of SUR1 and CSH1 caused an increase in the phosphorylated form of Slt2, a mitogen-activated protein kinase activated through impairment of CWI. Moreover, the deletion of SLT2 or WSC1/2, encoding a sensor protein recognizing CWI, enhanced the growth defect in the ERG9-repressed sur1∆ csh1∆ cells. On the other hand, the ERG9-repressed sur1∆ csh1∆ cells also exhibited an increase in the cell wall chitin level in a Slt2- and Wsc1/2-independent manner....Continue Reading

References

Jan 2, 1992·Gene·T W ChristiansonP Hieter
Jun 1, 1995·Journal of Bacteriology·J M van der VaartC T Verrips
Dec 31, 1997·The Journal of Biological Chemistry·D HaakT Dunn
Mar 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·M BagnatK Simons
Aug 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·K ThevissenW F Broekaert
Jan 30, 2002·Trends in Molecular Medicine·Antonella LupettiSteven Kelly
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Stefan Hohmann
Sep 5, 2003·The Journal of Biological Chemistry·Satoshi UemuraYasuyuki Igarashi
Mar 15, 2006·Molecular Genetics and Genomics : MGG·Elke EricsonAnders Blomberg
May 30, 2006·Progress in Lipid Research·Robert C DicksonRobert L Lester
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Guillaume Lesage, Howard Bussey
Mar 27, 2007·Cellular Microbiology·Daniel E Voth, Robert A Heinzen
Sep 27, 2007·Journal of Cell Science·Christopher TiedjeThomas Höfken
Sep 29, 2009·The Journal of Biological Chemistry·Isabelle E J A FrançoisKarin Thevissen
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Françoise M RoelantsJeremy Thorner
Jul 24, 2010·Molecular Genetics and Genomics : MGG·Sabrina WilkJürgen J Heinisch
Oct 6, 2010·Advances in Experimental Medicine and Biology·Robert C Dickson
Dec 4, 2010·The Journal of Biological Chemistry·Francisco Aresta-BrancoRodrigo F M de Almeida
May 18, 2011·Traffic·Michal A SurmaKai Simons
Jun 2, 2011·Cold Spring Harbor Perspectives in Biology·Kai Simons, Julio L Sampaio
Jan 11, 2012·Biochimica Et Biophysica Acta·Michal A SurmaKai Simons

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Citations

Sep 11, 2019·FEMS Microbiology Reviews·Alexandros AthanasopoulosChristos Gournas
Nov 4, 2020·FEBS Letters·Filipa C SantosRodrigo F M de Almeida
Feb 10, 2021·Journal of Cell Science·Alexander Mela, Michelle Momany
Apr 30, 2021·Journal of Cell Science·Alexander Mela, Michelle Momany
Jun 2, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Gabriel S MatosMonica Montero-Lomelí
Jul 31, 2021·Critical Reviews in Biotechnology·Ge ZhangZongjie Dai

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