Manual Collection and Semen Characterization in a West Indian Manatee (Trichechus manatus )

Frontiers in Veterinary Science
Jonathan R CowartIskande V Larkin

Abstract

Limited information is available regarding male reproductive physiology in West Indian manatees (Trichechus manatus). Currently, only basic ultrastructural and morphometric descriptions of the spermatozoon exist; however, there are no reports evaluating any seminal characteristics in this species. Therefore, the aim of the study was to fill current gaps in knowledge regarding semen parameters in West Indian manatees by collecting and characterizing multiple ejaculate samples from a single, adult West Indian manatee. Samples were analyzed for the following semen parameters: volume, agglutination, pH, osmolality, viscosity, concentration, total sperm number, motility and kinematic parameters, morphology, plasma membrane integrity, acrosome integrity, chromatin maturation, and chromatin condensation. All macroscopic semen parameters varied to some extent between samples. Total and progressive motility was high for ejaculates 2 to 5, exceeding 97 and 89%, respectively; however, these parameters decreased dramatically throughout ejaculates 6 and 7. Across all samples, curvilinear velocity, straight-line velocity, and average pathway velocity represented the largest significant differences (p < 0.001) between each of the progression ...Continue Reading

References

May 1, 1992·Journal of Reproduction and Fertility·A J RitarI G White
Mar 26, 1999·Molecular Reproduction and Development·J L Larson, D J Miller
Sep 6, 2001·Anatomia, histologia, embryologia·D L MillerG D Bossart
Jul 20, 2002·Physiology & Behavior·Nicholas PoundAlfonso P Del Valle
Jan 8, 2008·Fertility and Sterility·Alexander WongPhilip J Chan
Apr 25, 2013·Clinical and Experimental Reproductive Medicine·Hee-Sun KimYoung Min Choi

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Citations

Jun 3, 2021·Animals : an Open Access Journal From MDPI·Gerhard van der Horst

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Methods Mentioned

BETA
urine sampling
semen collection

Software Mentioned

SCA
R

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