Manual polishing of 3D printed metals produced by laser powder bed fusion reduces biofilm formation

PloS One
Marissa McGaffeyJ Scott Weese

Abstract

Certain 3D printed metals and surface finishes may be better suited for canine patient specific orthopedic implants on the basis of minimizing potential bacterial biofilm growth. Thirty disks each of titanium alloy, stainless steel, and cobalt chromium alloy were 3D printed via laser powder bed fusion. Fifteen disks of each metal were subsequently polished. After incubation with a robust biofilm-forming methicillin-resistant Staphylococcus pseudintermedius isolate, disks were rinsed and sonicated to collect biofilm bacteria. Serial dilutions were plated on blood agar, and colony forming units were counted log (ln) transformed for analysis of variance. Interference microscopy quantified surface roughness for comparison to biofilm growth. Scanning electron microscopy on both pre- and post-sonicated disks confirmed biofilm presence and subsequent removal, and visualized surface features on cleaned disks. Significantly more bacteria grew on rough versus polished metal preparations (p < 0.0001). Titanium alloy had more bacterial biofilm growth compared to cobalt chromium alloy (p = 0.0001) and stainless steel (p < 0.0001). There were no significant growth differences between cobalt chromium alloy and stainless steel (p = 0.4737). Re...Continue Reading

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Citations

Oct 23, 2019·Journal of Periodontal Research·Erica D de AvilaJeroen J J P van den Beucken
Nov 17, 2020·Journal of Oral Microbiology·Lukasz PalkaKatarzyna Baldy-Chudzik
Mar 24, 2021·Journal of Biomedical Materials Research. Part a·Bijan AbarSamuel B Adams
Apr 4, 2021·International Journal of Environmental Research and Public Health·Adele EvansKathryn A Whitehead

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Methods Mentioned

BETA
scanning electron microscopy

Software Mentioned

SAS
StatTrek

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