Manufacturing and ambient stability of shelf freeze dried bacteriophage powder formulations

International Journal of Pharmaceutics
Yajie ZhangDebadyuti Ghosh

Abstract

The severity of multidrug resistance to antibiotics has urged development of alternative treatment approaches, including bacteriophage therapy. Given the complexity of the bacteriophage structure, formulation and stability are primary concerns. Our present work optimized process and formulations of phage powder manufacturing and investigated the stability of lyophilized bacteriophage powders under ambient storage. The model phage M13 was formulated with trehalose, mannitol, sucrose and PEG6000 and lyophilized in different conditions. Bacteriophage viability was examined by titering and was considered as the assessment of phage stability. Less titer loss of trehalose and sucrose formulations were observed compared to mannitol and PEG groups both immediately after lyophilization and upon long term storage. When evaluating lyophilization conditions, an additional 1 log titer was preserved by reduction of product drying stress. Trehalose was stabilized in the amorphous state whereas mannitol stayed in crystalline state in lyophilized powders. Increased moisture content was demonstrated to have a positive impact on viability of phage after lyophilization and upon storage. Overall, 2% trehalose or sucrose (w/v) can sufficiently stabi...Continue Reading

Citations

Jan 10, 2019·Pest Management Science·Kelvin K KeringHongping Wei
Jul 20, 2019·Expert Review of Anti-infective Therapy·Ewa Jończyk-MatysiakAndrzej Górski
May 17, 2019·Journal of Clinical Microbiology·Katherine M Caflisch, Robin Patel
Apr 6, 2019·Frontiers in Microbiology·Alvin LyWarren H Finlay
May 23, 2020·Bioengineering & Translational Medicine·Rachel Yoon Kyung ChangHak-Kim Chan
May 1, 2021·Pharmaceuticals·Daniel Rosner, Jason Clark
Aug 29, 2018·Bioconjugate Chemistry·Raymond P WelchJeremiah J Gassensmith

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.