Manure Enhances Plasmid Mobilization and Survival of Pseudomonas putida Introduced into Field Soil.

Applied and Environmental Microbiology
A Götz, Kornelia Smalla

Abstract

We report a field study on plasmid mobilization in an agricultural soil. The influence of pig manure on the mobilization of the IncQ plasmid pIE723 by indigenous plasmids or by the IncP(alpha) plasmid pGP527 into the recipient Pseudomonas putida UWC1 (Rif(supr) Nal(supr)) was studied in field soil. Six plots were prepared in duplicate, three of which were treated with manure prior to inoculation of the donor and recipient strains. As a donor strain, either Escherichia coli J53(pIE723) or E. coli 600(pIE723, pGP527) was used. Putative transconjugants obtained on a selective medium were confirmed by DNA hybridization and PCR. Plasmid mobilization by indigenous mobilizing plasmids was observed on two occasions in manured soil. Manuring of soil significantly enhanced the frequency of pIE723 mobilization by pGP527, since mobilization frequencies into P. putida UWC1 were at least 10-fold higher in manured soil than in nonmanured soil. Enhanced numbers of P. putida UWC1 transconjugant and recipient colonies could be observed in manured soil throughout the 79-day field test. Transfer of pIE723 or pG527 into indigenous soil or rhizosphere bacteria could not be detected when indigenous bacteria isolated by selective cultivation were scre...Continue Reading

References

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Citations

Jun 19, 2008·FEMS Microbiology Ecology·Chu Thi Thanh BinhKornelia Smalla
Apr 29, 2009·Journal of Environmental Quality·Joanne C Chee-SanfordRustam I Aminov
Nov 1, 2002·FEMS Microbiology Ecology·Jan Dirk van Elsas, Mark J Bailey
Feb 9, 2012·Journal of Applied Microbiology·A L BarkovskiiC Bridges
Jan 8, 2015·Frontiers in Microbiology·Uli KlümperBarth F Smets
Jun 4, 2002·Nature Biotechnology·Philip J DaleEliana M G Fontes
Feb 13, 2008·Antimicrobial Agents and Chemotherapy·Carlos RevillaFernando de la Cruz
Nov 1, 2002·FEMS Microbiology Ecology·H HeuerK Smalla
Mar 8, 2012·FEMS Microbiology Reviews·Holger Heuer, Kornelia Smalla
Apr 15, 2011·Journal of Environmental Quality·Mariya Munir, Irene Xagoraraki
Apr 17, 2010·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Jiewen GuanJ Lloyd Spencer
Oct 7, 2004·Canadian Journal of Microbiology·A GorissenJ D van Elsas
Jul 14, 2010·Foodborne Pathogens and Disease·Spencer HeringaXiuping Jiang
Jul 6, 2000·Applied and Environmental Microbiology·A M HohnstockE L Madsen
Oct 27, 2007·Environmental Biosafety Research·Holger Heuer, Kornelia Smalla
Mar 7, 2014·Environmental Microbiology Reports·Sanin MusovicBarth F Smets
May 14, 2017·Frontiers of Medicine·Yongfei HuBaoli Zhu
Apr 21, 2019·Water Environment Research : a Research Publication of the Water Environment Federation·Qing-Bin YuanNan Hu
Jun 27, 2019·Microorganisms·Susanne A KraemerGabriel G Perron

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