Abstract
Reactive oxygen species (ROS) are a continuous hazard in eukaroytic cells by their ability to cause damage to biomolecules, in particular to DNA. Previous data indicated that the cytosolic serine peptidase tripeptidyl-peptidase II (TPPII) translocates into the nucleus of most tumor cell lines in response to gamma-irradiation and ROS production; an event that promoted p53 expression as well as caspase-activation. We here observed that nuclear translocation of TPPII was dependent on signaling by MAP kinases, including p38MAPK. Further, this was caused by several types of DNA-damaging drugs, a DNA cross-linker (cisplatinum), an inhibitor of topoisomerase II (etoposide), and to some extent also by nucleoside-analogues (5-fluorouracil, hydroxyurea). In the minority of tumor cell lines where TPPII was not translocated into the nucleus in response to DNA damage we observed reduced intracellular ROS levels, and the expression levels of redox defense systems were increased. Further, treatment with the ROS-inducer gamma-hexa-chloro-cyclohexane (gamma-HCH, lindane), an inhibitor of GAP junctions, restored nuclear translocation of TPPII in these cell lines upon gamma-irradiation. Moreover, blocking nuclear translocation of TPPII in etoposi...Continue Reading
References
May 2, 1997·The Journal of Biological Chemistry·P R YoungJ C Lee
Feb 12, 1999·Science·E GeierG Niedermann
Dec 3, 1999·The EMBO Journal·D V BulavinA J Fornace
Oct 2, 2001·American Journal of Medical Genetics·S Raha, B H Robinson
Jun 18, 2003·The Journal of Experimental Medicine·Xu HongRickard Glas
Aug 5, 2003·BioFactors·Martine Torres, Henry Jay Forman
Dec 22, 2004·Mechanisms of Ageing and Development·T von ZglinickiS P Jackson
May 13, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Nathalie LediracRoger Rahmani
Jul 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Beate RockelWolfgang Baumeister
Jun 30, 2006·Annual Review of Genetics·Jacob C Harrison, James E Haber
Feb 13, 2007·Cancer Cell·H Christian ReinhardtMichael B Yaffe
May 8, 2007·Biochimica Et Biophysica Acta·Ana Cuenda, Simon Rousseau
Jul 13, 2007·Trends in Biochemical Sciences·Jiahuai Han, Peiqing Sun
Dec 11, 2007·Oncogene·J BartekJ Lukas
Jul 4, 2008·Histochemistry and Cell Biology·John M Robinson
Nov 5, 2008·Cancer Biology & Therapy·Jie Wang, Jing Yi
Dec 17, 2008·Annals of the New York Academy of Sciences·Anatoly A Starkov
Jan 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Bettina GruhneMaria G Masucci
Jan 23, 2009·International Journal of Biological Sciences·Tina M Thornton, Mercedes Rincon
Feb 24, 2009·Current Opinion in Cell Biology·H Christian Reinhardt, Michael B Yaffe
Apr 9, 2009·Cancer Research·Elke FiratGabriele Niedermann
Apr 11, 2009·World Journal of Gastroenterology : WJG·Jin-Shui PanJian-Lin Ren
Sep 15, 2009·Biochemical and Biophysical Research Communications·Giulio PretaRickard Glas
Citations
Mar 31, 2012·Molecules and Cells·Hyojung PaikDoheon Lee
Jul 3, 2013·Acta histochemica·Giuseppe MusumeciPaola Castrogiovanni
Jul 21, 2011·Biochimica Et Biophysica Acta·Beate RockelWolfgang Baumeister
Jun 5, 2015·Molecular & Cellular Proteomics : MCP·Anne WiemhoeferEric A Reits
Jul 24, 2020·Computational Biology and Chemistry·Jarmila Nahálková