Map of physical interactions between extracellular domains of Arabidopsis leucine-rich repeat receptor kinases

Scientific Data
G Adam MottYoussef Belkhadir

Abstract

Plants use surface receptors to perceive information about many aspects of their local environment. These receptors physically interact to form both steady state and signalling competent complexes. The signalling events downstream of receptor activation impact both plant developmental and immune responses. Here, we present a comprehensive study of the physical interactions between the extracellular domains of leucine-rich repeat receptor kinases (LRR-RKs) in Arabidopsis. Using a sensitized assay, we tested reciprocal interactions among 200 of the 225 Arabidopsis LRR-RKs for a total search space of 40,000 interactions. Applying a stringent statistical cut-off and requiring that interactions performed well in both bait-prey and prey-bait orientations resulted in a high-confidence set of 567 bidirectional interactions. Additionally, we identified a total of 2,586 unidirectional interactions, which passed our stringent statistical cut-off in only one orientation. These datasets will guide further investigation into the regulatory roles of LRR-RKs in plant developmental and immune signalling decisions.

References

Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·S H Shiu, A B Bleecker
Jan 1, 2004·Development·Anne Diévart, Steven E Clark
Jan 9, 2004·Journal of Biomolecular Screening·Christine BrideauAndy Liaw
Apr 28, 2004·Journal of Molecular Biology·Lukas KällErik L L Sonnhammer
Feb 9, 2006·Nature Biotechnology·Nathalie MaloRobert Nadon
Feb 26, 2008·Genome Research·K Mark BushellGavin J Wright
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Nov 16, 2013·Nucleic Acids Research·Sandra OrchardHenning Hermjakob
Nov 19, 2013·Trends in Plant Science·Thomas W H LiebrandMatthieu H A J Joosten
Jan 7, 2014·Current Biology : CB·Thierry HalterBirgit Kemmerling
Aug 5, 2014·Trends in Biochemical Sciences·Youssef BelkhadirJoanne Chory
Dec 3, 2016·Nucleic Acids Research·Robert D FinnAlex L Mitchell
Jul 18, 2017·Current Opinion in Plant Biology·Rebecca C Burkart, Yvonne Stahl
Jan 11, 2018·Nature·Elwira Smakowska-LuzanYoussef Belkhadir
Jun 15, 2018·Nature Communications·Hadia AhmedM Shahid Mukhtar

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Citations

Oct 17, 2021·Journal of Experimental Botany·Emma K Turley, J Peter Etchells

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Software Mentioned

FigShare
Platero
SignalP4
TMHMM
InterPro
Phobius
AVEXIS

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