MAPK-dependent regulation of IL-1- and beta-adrenoreceptor-induced inflammatory cytokine production from mast cells: implications for the stress response

BMC Immunology
David S ChiGuha Krishnaswamy

Abstract

Catecholamines, such as epinephrine, are elaborated in stress responses, and mediate vasoconstriction to cause elevation in systemic vascular resistance and blood pressure. Our previous study has shown that IL-1 can induce mast cells to produce proinflammatory cytokines which are involved in atherogenesis. The aim of this study was to determine the effects of epinephrine on IL-1-induced proatherogenic cytokine production from mast cells. Two ml of HMC-1 (0.75 x 106 cells/ml) were cultured with epinephrine (1 x 10-5 M) in the presence or absence of IL-1 beta (10 ng/ml) for 24 hrs. HMC-1 cultured alone produced none to trace amounts of IL-6, IL-8, and IL-13. IL-1 beta significantly induced production of these cytokines in HMC-1, while epinephrine alone did not. However, IL-6, IL-8, and IL-13 production induced by IL-1 beta were significantly enhanced by addition of epinephrine. The enhancing effect appears to involve NF-kappa B and p38 MAPK pathways. Flow cytometry showed the presence of beta1 and beta2 adrenoreceptors on resting mast cells. The enhancing effect of proatherogenic cytokine production by epinephrine was down regulated by the beta1 and beta2 adrenoceptor antagonist, propranolol, but not by the beta1 adrenoceptor ant...Continue Reading

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Citations

May 20, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Ana Paula C DavelLuciana V Rossoni
Feb 13, 2018·Immunological Reviews·Hanna BonnekohKaroline Krause
Nov 5, 2016·British Journal of Pharmacology·Nadia Ayala-Lopez, Stephanie W Watts

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Methods Mentioned

BETA
ELISA
flow cytometry
PCR
Assay
X-ray
Electrophoresis
transfection
transfections

Software Mentioned

Statistica
WinMDI
Scan
It
Un

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