MAPK Pathway Involved in Epidermal Terminal Differentiation of Normal Human Epidermal Keratinocytes

Open Medicine
Xianguang MengNingning Dang

Abstract

To investigate the effect of mitogen-activated protein kinase (MAPK) signaling pathway in epidermal terminal differentiation. The MAPK pathways (p38, ERK1/2, JNK) were inhibited by SB203580, PD98059, and SP600125 in normal human epidermal keratinocytes (NHEKs), respectively. Western blotting assays were performed to detect expression of filaggrin and differentiation-related proteins. The mRNA expressions of differentiation-related proteins were detected by real-time quantitative PCR (qRT-PCR). Inhibition of MAPK pathway by SB203580, PD98059, and SP600125 resulted in significant reduction of filaggrin expression in NHEKs. Inhibition of the p38 MAPK pathway decreased the expression of differentiation-related proteins (cytokeratin 5, cytokeratin 14, ST14, and SPRR3), Akt, and NF-κB. Inhibition of JNK also suppressed expression of cytokeratin 14, SPRR3, Akt, and NF-κB. However, inhibition of ERK1/2 merely decreased expression of SPRR3 and Akt. MAPK pathways regulates epidermal terminal differentiation in NHEKs. The p38 signaling pathway plays an especially important role.

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Citations

Feb 9, 2020·International Journal of Molecular Sciences·Georgia NikoloudakiDouglas W Hamilton
Mar 30, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·So-Hyeon HwangJae Youl Cho
Jul 17, 2021·The Journal of Allergy and Clinical Immunology·Stanley B DeVoreGurjit K Khurana Hershey

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