MAPK phosphorylation-induced stabilization of ACS6 protein is mediated by the non-catalytic C-terminal domain, which also contains the cis-determinant for rapid degradation by the 26S proteasome pathway

The Plant Journal : for Cell and Molecular Biology
Sunjoo JooShuqun Zhang

Abstract

Ethylene is an important hormone in plant growth, development and responses to environmental stimuli. The ethylene-signaling pathway is initiated by the induction of ethylene biosynthesis, which is under tight regulation at both transcriptional and post-transcriptional levels by exogenous and endogenous cues. 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is the rate-limiting enzyme that catalyzes the committing step of ethylene biosynthesis. Recently, we found that ACS2 and ACS6, two isoforms of the Arabidopsis ACS family, are substrates of a stress-responsive mitogen-activated protein kinase (MAPK) cascade. Phosphorylation of ACS2/ACS6 by MPK6 leads to the accumulation of ACS proteins and the induction of ethylene. In this report, we demonstrate that unphosphorylated ACS6 protein is rapidly degraded by the 26S proteasome pathway. The degradation machinery targets the C-terminal non-catalytic domain of ACS6, which is sufficient to confer instability to green fluorescent protein and luciferase reporters. Phosphorylation of ACS6 introduces negative charges to the C-terminus of ACS6, which reduces the turnover of ACS6 by the degradation machinery. Consistent with this, other nearby conserved negatively charged amino acid re...Continue Reading

References

Dec 1, 1994·Plant Molecular Biology·T I Zarembinski, A Theologis
Jan 1, 1997·Trends in Biotechnology·T MizoguchiK Shinozaki
May 1, 1997·The Plant Cell·S Zhang, D F Klessig
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·J P VogelJ J Kieber
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Jun 3, 2000·Annual Review of Immunology·M Karin, Y Ben-Neriah
Oct 14, 2000·Annual Review of Cell and Developmental Biology·A B Bleecker, H Kende
Nov 18, 2000·Plant Physiology·G FelixT Boller
Jan 12, 2001·Plant Physiology·H Kende
Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·K Y YangS Zhang
Jun 8, 2001·Annual Review of Biochemistry·C M Pickart
Oct 13, 2001·Current Opinion in Plant Biology·G TenaJ Sheen
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·S UsamiY Machida
Nov 10, 2001·Trends in Plant Science·S Zhang, D F Klessig
Jan 10, 2002·Cell·R J Deshaies, J E Ferrell
Jun 5, 2002·The Plant Cell·Kevin L-C WangJoseph R Ecker
Jul 18, 2002·Trends in Plant Science·UNKNOWN MAPK Group
Sep 12, 2003·The Journal of Biological Chemistry·Takeshi YamagamiAthanasios Theologis
Dec 3, 2003·The Journal of Biological Chemistry·Han C DanJin Q Cheng
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Atsunari Tsuchisaka, Athanasios Theologis
May 1, 2004·Nature·Kevin L-C WangJoseph R Ecker
Jun 23, 2004·Plant Physiology·Harry J Klee
Sep 21, 2004·Annual Review of Plant Biology·Jan Smalle, Richard D Vierstra
Jun 14, 2005·Trends in Plant Science·Hyun Sook Chae, Joseph J Kieber
Jun 15, 2005·Trends in Plant Science·Hirofumi NakagamiHeribert Hirt
Jul 27, 2005·Current Opinion in Plant Biology·Kerry F Pedley, Gregory B Martin
Dec 13, 2005·Methods in Enzymology·Xiaojing TangMike Tyers
Apr 8, 2006·Cell Death and Differentiation·M F Lavin, N Gueven
Apr 11, 2006·Annual Review of Phytopathology·Willem F BroekaertBruno P A Cammue

❮ Previous
Next ❯

Citations

May 9, 2009·Nature Reviews. Molecular Cell Biology·Richard D Vierstra
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Gerit BethkeJustin Lee
Jul 2, 2009·Journal of Experimental Botany·Zhefeng LinDon Grierson
Mar 21, 2013·The Plant Cell·Gyeong Mee Yoon, Joseph J Kieber
May 15, 2013·Annual Review of Phytopathology·Xiangzong Meng, Shuqun Zhang
Apr 26, 2014·Molecular Plant-microbe Interactions : MPMI·Jian-Zhong LiuSteven A Whitham
Mar 5, 2011·Annual Review of Plant Biology·Zhihua Hua, Richard D Vierstra
Oct 27, 2015·The New Phytologist·Boyoung LeeChoong-Min Ryu
Feb 13, 2016·Frontiers in Plant Science·Els KeunenAnn Cuypers
Jun 23, 2015·Plant Biotechnology Journal·Fangfang MaMingyi Jiang
Jan 6, 2010·Trends in Plant Science·Erik Andreasson, Brian Ellis
Sep 1, 2009·Current Opinion in Plant Biology·Berthe Katrine FiilJohn Mundy
Apr 28, 2009·The Plant Journal : for Cell and Molecular Biology·Ankit WaliaBrian Ellis
Feb 3, 2009·Plant, Cell & Environment·Jianqiang Wu, Ian T Baldwin
Aug 21, 2008·Journal of Integrative Plant Biology·Ziqiang Zhu, Hongwei Guo
Sep 24, 2008·The Plant Journal : for Cell and Molecular Biology·Matthew J ChristiansRichard D Vierstra
Nov 5, 2008·The Plant Journal : for Cell and Molecular Biology·Maureen HansenJoseph J Kieber
Feb 13, 2009·Proteomics·Birgit KerstenRandeep Rakwal
Mar 1, 2013·Journal of the Science of Food and Agriculture·Ruirui ZhaoJiping Sheng
Jul 28, 2010·The Plant Journal : for Cell and Molecular Biology·Yusuke KamiyoshiharaHitoshi Mori
Jul 28, 2010·The Plant Journal : for Cell and Molecular Biology·Ling HanShuqun Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.