Mapping activation and repression domains of the vnd/NK-2 homeodomain protein

Proceedings of the National Academy of Sciences of the United States of America
Alexander Stepchenko, Marshall Nirenberg

Abstract

A transient transfection assay using Drosophila S2 tissue culture cells and WT and mutant Drosophila vnd/NK-2 homeobox cDNAs was used to localize repression and activation domains of vnd/NK-2 homeodomain protein. A repression domain was identified near the N terminus of vnd/NK-2 homeodomain protein (amino acid residues 154-193), which contains many hydrophobic amino acid residues. The major determinants of the repression domain were shown to be amino acid residues F155, W158, I161, L162, L163, and W166. Truncated protein consisting of the N-terminal repression domain and the DNA-binding homeodomain repressed transcription as efficiently as WT vnd/NK-2 protein. An activation domain was identified between the tinman domain and the homeodomain (amino acid residues 277-543), which consists of a glutamine-rich subdomain and two acidic subdomains. No effect was detected of the tinman domain or the NK-2-specific domain on either activation or repression of a beta-galactosidase reporter gene.

References

Jul 26, 1990·Nature·M Ptashne, A A Gann
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y Kim, M Nirenberg
Nov 3, 1995·The Journal of Biological Chemistry·M De FeliceR Di Lauro
Jun 30, 1995·Annals of the New York Academy of Sciences·M NirenbergR Lad
Apr 1, 1994·Molecular and Cellular Biology·C E DabrowskiP A Schaffer
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A BaniahmadB W O'Malley
Apr 29, 1993·Biochimica Et Biophysica Acta·C P Verrijzer, P C Van der Vliet
Mar 18, 1996·Biochemical and Biophysical Research Communications·E Wey, B W Schäfer
Apr 1, 1996·Current Opinion in Genetics & Development·J A Ranish, S Hahn
Aug 1, 1996·Trends in Genetics : TIG·L A Stargell, K Struhl
Aug 1, 1996·Mechanisms of Development·M D'Alessio, M Frasch
May 7, 1998·The International Journal of Biochemistry & Cell Biology·L LaniaP De Luca
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·B XiangJ A Ferretti
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·H H SaundersM Nirenberg
Sep 30, 1998·Cell·S C West
Jun 5, 1999·Journal of Molecular Biology·J M GruschusJ A Ferretti
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·H WatadaM S German
Jul 20, 2002·Mechanisms of Development·Jay UhlerDervla M Mellerick
Sep 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lan-Hsiang WangMarshall Nirenberg

❮ Previous
Next ❯

Citations

Mar 14, 2007·Molecular and Cellular Biology·Hui-Yi Chu, Akihira Ohtoshi
May 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Lan-Hsiang WangMarshall Nirenberg
Sep 29, 2006·Developmental Biology·Pliny A Smith, Susan E Mango
May 11, 2005·Developmental Biology·Angelike Stathopoulos, Michael Levine

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.