Jan 6, 2016

Mapping challenging mutations by whole-genome sequencing

BioRxiv : the Preprint Server for Biology
Harold E SmithAndy Golden

Abstract

Whole-genome sequencing provides a rapid and powerful method for identifying mutations on a global scale, and has spurred a renewed enthusiasm for classical genetic screens in model organisms. The most commonly characterized category of mutation consists of monogenic, recessive traits, due to their genetic tractability. Therefore, most of the mapping methods for mutation identification by whole-genome sequencing are directed toward alleles that fulfill those criteria (i.e., single-gene, homozygous variants). However, such approaches are not entirely suitable for the characterization of a variety of more challenging mutations, such as dominant and semi-dominant alleles or multigenic traits. Therefore, we have developed strategies for the identification of those classes of mutations, using polymorphism mapping in Caenorhabditis elegans as our model for validation. We also report an alternative approach for mutation identification from traditional recombinant crosses, and a solution to the technical challenge of sequencing sterile or terminally arrested strains where population size is limiting. The methods described herein extend the applicability of whole-genome sequencing to a broader spectrum of mutations, including classes th...Continue Reading

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Mentioned in this Paper

Gene Polymorphism
Size
Genes
Caenorhabditis elegans
Genetic Screening (Procedure)
Genetic Activator
Sequencing
dominant allele
Recombinants
Infertility

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