PMID: 7522964Sep 1, 1994Paper

Mapping effector functions of a monoclonal antibody to GD3 by characterization of a mouse-human chimeric antibody

Cancer Immunology, Immunotherapy : CII
P B ChapmanR M Reilly

Abstract

R24, a mouse monoclonal antibody against GD3 ganglioside, exhibits a wide range of in vitro effector functions. It also has the ability to bind to itself, presumably through homophilic Fab-Fab interactions, which have been proposed to contribute to its high relative avidity for GD3 and to its effector function activity. It is not known which of these characteristics is necessary for the antitumor effects observed in melanoma patients treated with R24. A mouse-human chimeric R24 (chR24) molecule has been constructed in which the GD3-binding site is preserved. Chimeric R24 demonstrates a lower level of binding to GD3 than does mouse R24 suggesting that there may be some differences between the GD3-binding sites of the two mAb or that Fc determinants can contribute to R24 avidity for GD3. The property of homophilic binding is retained by chR24, demonstrating formally that homophilic binding of R24 involves interactions between variable domains. Both R24 and chR24 fix human complement and mediate antibody-dependent cellular cytotoxicity although chR24 was slightly less efficient at the latter. Unlike R24, chR24 was not able to inhibit melanoma cell attachment to plastic surfaces and was not able to activate human T lymphocytes. We ...Continue Reading

References

Jul 1, 1991·The Journal of Clinical Investigation·P B Chapman, A N Houghton
Dec 20, 1989·Journal of Immunological Methods·S D GilliesJ Wesolowski
Aug 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N S GreenspanL J Cooper
Mar 15, 1989·Journal of the National Cancer Institute·D IliopoulosD Herlyn
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A Y LiuI Hellström
Jun 15, 1988·Journal of the National Cancer Institute·G Hendricks
Oct 1, 1988·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S Vadhan-RajA N Houghton
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W G DippoldL J Old

❮ Previous
Next ❯

Citations

May 15, 2013·The American Journal of Pathology·Emily R GilbertI Caroline Le Poole
Sep 24, 2011·Seminars in Immunology·Matthew H Levine, Peter L Abt
Aug 21, 2013·Blood Reviews·Irit AviviTamar Katz
Sep 6, 2008·Advances in Immunology·Katherine A McLaughlin, Kai W Wucherpfennig
Dec 31, 2014·La Revue de médecine interne·G SauvètreY Benhamou
Nov 6, 2012·Advanced Drug Delivery Reviews·Jindřich Kopeček
Jul 20, 2016·Clinical Immunology : the Official Journal of the Clinical Immunology Society·William Stohl, Agnes Banfalvi
Jun 30, 2015·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Anette HellerAndrea S Bauer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.