Mapping Enterovirus A71 Antigenic Determinants from Viral Evolution

Journal of Virology
Sheng-Wen HuangJen-Ren Wang

Abstract

Human enterovirus A71 (EV-A71) belongs to the Enterovirus A species in the Picornaviridae family. Several vaccines against EV-A71, a disease causing severe neurological complications or even death, are currently under development and being tested in clinical trials, and preventative vaccination programs are expected to start soon. To characterize the potential for antigenic change of EV-A71, we compared the sequences of two antigenically diverse genotype B4 and B5 strains of EV-A71 and identified substitutions at residues 98, 145, and 164 in the VP1 capsid protein as antigenic determinants. To examine the effects of these three substitutions on antigenicity, we constructed a series of recombinant viruses containing different mutation combinations at these three residues with a reverse genetics system and then investigated the molecular basis of antigenic changes with antigenic cartography. We found that a novel EV-A71 mutant, containing lysine, glutamine, and glutamic acid at the respective residues 98, 145, and 164 in the VP1 capsid protein, exhibited neutralization reduction against patients' antisera and substantially increased virus binding ability to human cells. These observations indicated that this low-neutralization-re...Continue Reading

References

Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M ChowD Baltimore
Mar 1, 1971·The Journal of Hygiene·M S Pereira, G C Schild
Sep 25, 1999·The New England Journal of Medicine·M HoS R Shih
Jun 26, 2004·Science·Derek J SmithRon A M Fouchier
Jul 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Joost W H SchymkowitzLuis Serrano
Feb 9, 2007·Journal of Virology·J C de JongR A M Fouchier
Dec 14, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D G W FooC L Poh
Apr 19, 2008·Science·Colin A RussellDerek J Smith
Jun 23, 2009·Nature Medicine·Seiya YamayoshiSatoshi Koike
Jun 23, 2009·Nature Medicine·Yorihiro NishimuraHiroyuki Shimizu
Sep 25, 2009·Journal of Clinical Microbiology·Sheng-Wen HuangJen-Ren Wang
Dec 17, 2009·Vaccine·Ian G BarrUNKNOWN Writing Committee of the World Health Organization Consultation on Northern Hemisphere Influenza Vaccine Composition for 200
Mar 1, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Sabine van der SandenMarion Koopmans
Mar 3, 2012·Science·Pavel PlevkaMichael G Rossmann
Oct 15, 2013·Emerging Health Threats Journal·Cyril C Y YipKwok-Yung Yuen
Jul 6, 2014·Expert Opinion on Biological Therapy·Yu-An KungShin-Ru Shih

❮ Previous
Next ❯

Citations

Mar 25, 2016·PLoS Neglected Tropical Diseases·Nmn NikNadiaYoke Fun Chan
Mar 22, 2018·BMC Infectious Diseases·Jie LiQuanyi Wang
Sep 25, 2017·Scientific Reports·Eveliina KarelehtoKatja C Wolthers
Oct 22, 2019·Journal of Biomedical Science·Sheng-Wen HuangJen-Ren Wang
Jan 22, 2020·Emerging Infectious Diseases·Lam Anh NguyetLe Van Tan
May 10, 2017·BMC Infectious Diseases·Nghia Ngu DuyRoger Frutos
Oct 23, 2019·International Journal of Molecular Sciences·Gabriel GonzalezTsuguto Fujimoto
Feb 21, 2021·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Jingyi ZhouYongjuan Liu
Apr 11, 2021·Proceedings of the National Academy of Sciences of the United States of America·Joseph A KendraGabriel I Parra

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.