Mapping human serum-induced gene networks as a basis for the creation of biomimetic periosteum for bone repair.

Cytotherapy
Rawiya Al HosniScott J Roberts

Abstract

The periosteum is a highly vascularized, collagen-rich tissue that plays a crucial role in directing bone repair. This is orchestrated primarily by its resident progenitor cell population. Indeed, preservation of periosteum integrity is critical for bone healing. Cells extracted from the periosteum retain their osteochondrogenic properties and as such are a promising basis for tissue engineering strategies for the repair of bone defects. However, the culture expansion conditions and the way in which the cells are reintroduced to the defect site are critical aspects of successful translation. Indeed, expansion in human serum and implantation on biomimetic materials has previously been shown to improve in vivo bone formation. This study aimed to develop a protocol to allow for the expansion of human periosteum derived cells (hPDCs) in a biomimetic periosteal-like environment. The expansion conditions were defined through the investigation of the bioactive cues involved in augmenting hPDC proliferative and multipotency characteristics, based on transcriptomic analysis of cells cultured in human serum. Master regulators of transcriptional networks were identified, and an optimized periosteum-derived growth factor cocktail (PD-GFC; ...Continue Reading

References

Jan 1, 1992·Pharmacology & Therapeutics·U Bjare
Apr 13, 2001·Osteoarthritis and Cartilage·Y ItoS W O'Driscoll
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 25, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J van der ValkV Baumans
May 29, 2004·Tissue Engineering·Jan De BoerClemens Van Blitterswijk
Feb 5, 2005·World Journal of Surgical Oncology·Ulf R Knothe, Dempsey S Springfield
May 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ramakrishnaiah SiddappaJan de Boer
Oct 14, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Céline Colnot
Jan 9, 2010·Journal of Tissue Engineering and Regenerative Medicine·Yanling XiaoJoost D de Bruijn
Apr 15, 2010·ALTEX·Daniel BrunnerGerhard Gstraunthaler
Jun 3, 2010·Cold Spring Harbor Protocols·Donald C RioTimothy W Nilsen
Aug 3, 2010·Journal of Biomechanics·Ulf R KnotheMelissa L Knothe Tate
Aug 13, 2010·Nature·Simón Méndez-FerrerPaul S Frenette
Sep 9, 2010·PLoS Computational Biology·Liesbet GerisHans Van Oosterwyck
Jan 1, 2010·Journal of Long-term Effects of Medical Implants·Peter Petrochenko, Roger J Narayan
Sep 29, 2011·Tissue Engineering. Part a·Joyce DoornJan de Boer
Sep 7, 2012·Cell Cycle·Chih-Chien Tsai, Shih-Chieh Hung
Oct 4, 2013·Journal of Dental Research·Z LinG Intini
Nov 12, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·P S LienemannM Ehrbar
Feb 12, 2015·Development·Siim Pauklin, Ludovic Vallier
Nov 2, 2016·Frontiers in Cell and Developmental Biology·Marina MontaliSimone Pacini
Mar 10, 2017·Scientific Reports·Tarig MagdeldinUmber Cheema
Aug 12, 2017·ALTEX·Jan van der ValkGerhard Gstraunthaler
Feb 24, 2018·Nature Communications·Oriane Duchamp de LagenesteCéline Colnot
Sep 27, 2018·Nature·Shawon DebnathMatthew B Greenblatt
Oct 16, 2018·Integrative Biology : Quantitative Biosciences From Nano to Macro·Juan WangIan C Schneider

❮ Previous
Next ❯

Citations

May 4, 2021·Advanced Healthcare Materials·Yiting LouBinbin Ying
Dec 18, 2021·Scientific Reports·Deniz BakkalciUmber Cheema

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

© 2022 Meta ULC. All rights reserved