Mapping individual cosmid DNAs by direct AFM imaging

Genomics
D P AllisonR J Warmack

Abstract

Individual cosmid clones have been restriction mapped by directly imaging, with the atomic force microscope (AFM), a mutant EcoRI endonuclease site-specifically bound to DNA. Images and data are presented that locate six restriction sites, predicted from gel electrophoresis, on a 35-kb cosmid isolated from mouse chromosome 7. Measured distances between endonuclease molecules bound to lambda DNA, when compared to known values, demonstrate the accuracy of AFM mapping to better than 1%. These results may be extended to identify other important site-specific protein-DNA interactions, such as transcription factor and mismatch repair enzyme binding, difficult to resolve by current techniques.

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Citations

Apr 28, 2001·Annual Review of Physical Chemistry·H G Hansma
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·L I PietrasantaH Hansma
Aug 7, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Claudio Rivetti
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