PMID: 9166587Jun 1, 1997Paper

Mapping of 22 expressed sequence tags isolated from a porcine small intestine cDNA library

Mammalian Genome : Official Journal of the International Mammalian Genome Society
C B JorgensenM Fredholm

Abstract

Complementary DNA sequences were selected from a resource of tentatively identified clones from a porcine small intestine cDNA library. Forty PCR primer pairs were designed to amplify 101-309 base pairs of the 3' untranslated region of the genes. The PCR conditions were optimized by altering both formamide and magnesium concentrations on samples of pig, mouse, and hamster DNA. Twenty primer pairs that, under stringent conditions, were pig-specific and amplified the expected fragments were chosen for regional assignment in a pig/rodent hybrid cell panel. Furthermore, 22 primer pairs were chosen to amplify DNA from the parental animals of the PiGMaP shared reference families in order to detect possible polymorphisms. Primer pairs that generated polymorphisms were used for genetic mapping. A total of 22 porcine expressed sequence tags (ESTs) were cytogenetically or genetically mapped by this approach. Twelve of the mapped ESTs could be added to the human-porcine comparative map.

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D R BeierD J Sussman
Mar 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A L ArchibaldA K Winterø
Mar 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M YerleA A Bosma
Feb 1, 1993·Nature Genetics·S J O'BrienN G Copeland
Apr 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·G RettenbergerH Hameister
Apr 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L FrönickeI Gustavsson
Jun 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A RobicJ Gellin
Jul 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A K WinteroW Davies
May 1, 1996·Genome Research·G A RohrerC W Beattie
Mar 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·D MilanJ Gellin
Oct 25, 1996·Science·G D SchulerT J Hudson
Jan 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·D MilanJ Gellin
Jan 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·P MarianiL Andersson
Feb 1, 1997·Computer Applications in the Biosciences : CABIOS·C ChevaletM SanCristobal-Gaudy

❮ Previous
Next ❯

Citations

Jul 25, 2003·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Susanna CireraMerete Fredholm
Dec 16, 2004·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Angela BarbosaChristine Renard
Aug 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M YerleJ Gellin
May 8, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A K WinteroM Fredholm
May 8, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·P D ThomsenM Fredholm
Mar 18, 2000·Animal Biotechnology·M YerleJ Gellin
Mar 31, 2009·BMC Genomics·Rikke K K VingborgChristian Bendixen
Dec 23, 2003·Animal Genetics·B J JungeriusM A M Groenen
May 19, 2004·Animal Genetics·M KopecnýL J Peelman
Dec 6, 2007·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·K WimmersS Ponsuksili
Jun 6, 2009·International Journal of Immunogenetics·K WimmersS Ponsuksili

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Mammalian Genome : Official Journal of the International Mammalian Genome Society
A K FridolfssonH Ellegren
Mammalian Genome : Official Journal of the International Mammalian Genome Society
P PintonMartine Yerle
Mammalian Genome : Official Journal of the International Mammalian Genome Society
P D ThomsenM Fredholm
Mammalian Genome : Official Journal of the International Mammalian Genome Society
M KojimaH Yasue
Mammalian Genome : Official Journal of the International Mammalian Genome Society
A K WinteroM Fredholm
© 2021 Meta ULC. All rights reserved